{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T08:52:25Z","timestamp":1791535945771,"version":"4.3.4"},"reference-count":55,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T00:00:00Z","timestamp":1448409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Euroimmun (Lubeck, Germany)"},{"name":"St. Vincent's Hospital IT Department and Research Endowment Foundation"},{"name":"Scleroderma Australia"},{"DOI":"10.13039\/501100000940","name":"Arthritis Australia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000940","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Actelion Australia"},{"DOI":"10.13039\/100004330","name":"GlaxoSmithKline","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004330","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the relationships between systemic sclerosis (SSc)\u2013related autoantibodies, as well as their clinical associations, in a well\u2010characterized Australian patient cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum from 505 Australian SSc patients were analyzed with a commercial line immunoassay (EuroLine; Euroimmun) for autoantibodies to centromere proteins CENP\u2010A and CENP\u2010B, RNA polymerase III (RNAP III; epitopes 11 and 155), the 90\u2010kd nucleolar protein NOR\u201090, fibrillarin, Th\/To, PM\/Scl\u201075, PM\/Scl\u2010100, Ku, topoisomerase I (topo I), tripartite motif\u2013containing protein 21\/Ro 52, and platelet\u2010derived growth factor receptor. Patient subgroups were identified by hierarchical clustering of the first 2 dimensions of a principal components analysis of quantitative autoantibody scores. Results were compared with detailed clinical data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 449 of the 505 patients were positive for at least 1 autoantibody by immunoblotting. Heatmap visualization of autoantibody scores, along with principal components analysis clustering, demonstrated strong, mutually exclusive relationships between CENP, RNAP III, and topo I. Five patient clusters were identified: CENP, RNAP III strong, RNAP III weak, topo I, and other. Clinical features associated with CENP, RNAP III, and topo I were consistent with previously published reports concerning limited cutaneous and diffuse cutaneous SSc. A novel finding was the statistical separation of RNAP III into 2 clusters. Patients in the RNAP III strong cluster had an increased risk of gastric antral vascular ectasia, but a lower risk of esophageal dysmotility. Patients in the other cluster were more likely to be male and to have a history of smoking and a history of malignancy, but were less likely to have telangiectasia, Raynaud's phenomenon, and joint contractures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Five major autoantibody clusters with specific clinical and serologic associations were identified in Australian SSc patients. Subclassification and disease stratification using autoantibodies may have clinical utility, particularly in early disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.39316","type":"journal-article","created":{"date-parts":[[2015,8,5]],"date-time":"2015-08-05T20:38:01Z","timestamp":1438807081000},"page":"3234-3244","source":"Crossref","is-referenced-by-count":109,"title":["Interpretation of an Extended Autoantibody Profile in a Well\u2010Characterized Australian Systemic Sclerosis (Scleroderma) Cohort Using Principal Components Analysis"],"prefix":"10.1002","volume":"67","author":[{"given":"K. A.","family":"Patterson","sequence":"first","affiliation":[{"name":"Flinders University, Bedford Park, South Australia, and Commonwealth Scientific and Industrial Research Organization (CSIRO) Adelaide  South Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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