{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T15:46:00Z","timestamp":1792856760306,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH (National Institute of Arthritis and Musculoskeletal and Skin Diseases Core Center)","award":["P30-AR-061271"],"award-info":[{"award-number":["P30-AR-061271"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH (National Institute of Arthritis and Musculoskeletal and Skin Diseases Core Center)","award":["K24-AR-060297"],"award-info":[{"award-number":["K24-AR-060297"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH (National Institute of Arthritis and Musculoskeletal and Skin Diseases Core Center)","award":["R01-AR-19616"],"award-info":[{"award-number":["R01-AR-19616"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To examine whether lung endothelial cells (ECs) from patients with systemic sclerosis (SSc)\u2013associated interstitial lung disease (ILD) express mesenchymal cell\u2013specific proteins and gene transcripts, indicative of the occurrence of endothelial\u2010to\u2010mesenchymal phenotypic transition (EndoMT).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Lung tissue from 6 patients with SSc\u2010associated pulmonary fibrosis was examined by histopathology and immunohistochemistry. Confocal laser microscopy was utilized to assess the simultaneous expression of EC and myofibroblast molecular markers. CD31+CD102+ ECs were isolated from the lung tissue of 2 patients with SSc\u2010associated ILD and 2 normal control subjects, and the expression of EC and mesenchymal cell markers and other relevant genes was analyzed by quantitative polymerase chain reaction, immunofluorescence microscopy, and Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Immunohistochemical staining revealed cells expressing the EC\u2010specific marker CD31 in the subendothelial, perivascular, and parenchymal regions of the lungs from all SSc patients. Confocal microscopy identified cells displaying simultaneous expression of von Willebrand factor and \u03b1\u2010smooth muscle actin in small and medium\u2010sized arterioles in the SSc lung tissue but not in normal control lungs. CD31+CD102+ ECs isolated from SSc lungs expressed high levels of mesenchymal cell\u2013specific genes (type I collagen, type III collagen, and fibronectin), EC\u2010specific genes (type IV collagen and VE\u2010cadherin), profibrotic genes (transforming growth factor \u03b21 and connective tissue growth factor), and genes encoding EndoMT\u2010related transcription factors (TWIST1 and SNAI2).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Cells coexpressing EC\u2010 and mesenchymal cell\u2013specific molecules are present in the lungs of patients with SSc\u2010associated ILD. CD31+CD102+ ECs isolated from SSc lungs simultaneously expressed mesenchymal cell\u2013 and EC\u2010specific transcripts and proteins. Collectively, these observations demonstrate the occurrence of EndoMT in the lungs of patients with SSc\u2010associated ILD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.39421","type":"journal-article","created":{"date-parts":[[2015,9,11]],"date-time":"2015-09-11T12:53:12Z","timestamp":1441975992000},"page":"210-217","source":"Crossref","is-referenced-by-count":114,"title":["Endothelial Cells Expressing Endothelial and Mesenchymal Cell Gene Products in Lung Tissue From Patients With Systemic Sclerosis\u2013Associated Interstitial Lung Disease"],"prefix":"10.1002","volume":"68","author":[{"given":"Fabian A.","family":"Mendoza","sequence":"first","affiliation":[{"name":"Thomas Jefferson University Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonsoles","family":"Piera\u2010Velazquez","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University Philadelphia 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