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Specific inhibition of this pathway is a core area for the development of new therapies for SLE. This study was undertaken to clarify the pathogenic mechanism involved and to identify new therapeutic targets, using a high\u2010throughput screening platform to determine novel regulators that contribute to the overactivation of the type I IFN signaling pathway in SLE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A high\u2010throughput IFN\u2010stimulated response element (ISRE)\u2013luciferase assay was used to screen for candidate genes that regulate the IFN signaling pathway. Western blotting was used to confirm the regulatory function of CDK1. SYBR Green quantitative reverse transcriptase\u2013polymerase chain reaction was used to detect the expression of individual IFN\u2010stimulated genes (ISGs). The differential expression of CDK1 and ISGs in SLE patients and healthy controls was analyzed using RNA sequencing data and a microarray.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The high\u2010throughput ISRE\u2010luciferase assay revealed that CDK1 enhanced type I IFN signaling. Consistent with this finding, CDK1 promoted the type I IFN\u2013induced phosphorylation of STAT\u20101 and the up\u2010regulated expression of ISGs. CDK1 expression was elevated in peripheral blood mononuclear cells (PBMCs) and kidney biopsy specimens from SLE patients and correlated positively with their IFN scores. A CDK1 inhibitor reduced the expression of ISGs in PBMCs from SLE patients and in renal cells from mice with lupus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate that CDK1 is a positive regulator of the IFN signaling pathway. The overexpression of CDK1 might contribute to the abnormally amplified type I IFN signaling in SLE, and the inhibition of CDK1 could be used to down\u2010regulate type I IFN signaling in SLE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.39543","type":"journal-article","created":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T11:00:44Z","timestamp":1450090844000},"page":"1222-1232","source":"Crossref","is-referenced-by-count":31,"title":["Identification of Cyclin\u2010Dependent Kinase 1 as a Novel Regulator of Type I Interferon Signaling in Systemic Lupus Erythematosus"],"prefix":"10.1002","volume":"68","author":[{"given":"Lingling","family":"Wu","sequence":"first","affiliation":[{"name":"Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Qin","sequence":"additional","affiliation":[{"name":"Shanghai Institute of Rheumatology, Renji 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