{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T21:17:14Z","timestamp":1783631834944,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T00:00:00Z","timestamp":1466726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"F. P. Fischer Stichting, Amersfoort"},{"name":"ODAS Stichting, Delft, the Landelijke Stichting Voor Blinden en Slechtzienden, Utrecht"},{"name":"Stichting Nederlands Oogheelkundig Onderzoek, Rotterdam, The Netherlands"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Childhood uveitis is a vision\u2010threatening inflammatory eye disease commonly attributed to juvenile idiopathic arthritis (JIA). The pathogenesis is poorly understood, which makes clinical management challenging. We analyzed soluble mediators in ocular fluid (aqueous humor [AqH]) and serum from children with JIA\u2010associated uveitis and common childhood uveitis to identify potential biomarkers and investigate the ocular microenvironment of this sight\u2010threatening eye disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>AqH (n\u2009=\u200973) and paired serum (n\u2009=\u200966) samples were analyzed for 51 soluble mediators of inflammation by multiplex immunoassay. Twenty\u2010one children with JIA\u2010associated uveitis were compared to 15 children with chronic anterior uveitis without arthritis, 29 children with noninfectious idiopathic uveitis, and 8 children with noninflammatory conditions (controls). For visualization of the joint effect of multiple mediators, we used the radial coordinate visualization (Radviz) method. Optimal biomarker level cutoffs were also determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The levels of interleukin\u201029 (IL\u201029)\/interferon\u2010\u03bb1 (IFN\u03bb1) were decreased (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and the levels of latency\u2010associated peptide and osteoprotegerin were increased (<jats:italic>P<\/jats:italic>\u2009=\u20090.002 and <jats:italic>P<\/jats:italic>\u2009=\u20090.001, respectively) in samples of AqH, but not serum, from patients with JIA\u2010associated uveitis. Multivariate analysis correcting for disease activity and treatment revealed that intraocular levels of IL\u201029\/IFN\u03bb1 were specifically decreased in patients with JIA\u2010associated uveitis as compared to those with idiopathic uveitis. Indeed, JIA\u2010associated uveitis patients and idiopathic uveitis patients showed distinct profiles of intraocular soluble mediators. IL\u201029\/IFN\u03bb1 showed a high area under the curve value (0.954), with 23.5 pg\/ml as the optimal cutoff value.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We identified IL\u201029\/IFN\u03bb1 as an intraocular biomarker for JIA\u2010associated uveitis, which suggests that aberrant IFN\u03bb signaling might be important in JIA\u2010associated uveitis and distinct from other forms of childhood uveitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.39621","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T09:20:44Z","timestamp":1455182444000},"page":"1769-1779","source":"Crossref","is-referenced-by-count":37,"title":["Ocular Fluid Analysis in Children Reveals Interleukin\u201029\/Interferon\u2010\u03bb1 as a Biomarker for Juvenile Idiopathic Arthritis\u2013Associated Uveitis"],"prefix":"10.1002","volume":"68","author":[{"given":"Anne\u2010Mieke J. W.","family":"Haasnoot","sequence":"first","affiliation":[{"name":"Utrecht University Medical Center Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas J. W.","family":"Kuiper","sequence":"additional","affiliation":[{"name":"Utrecht University Medical Center Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"Hiddingh","sequence":"additional","affiliation":[{"name":"Utrecht University Medical Center Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A. W. J. 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