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The features of the skin grafts were analyzed by immunohistochemistry, and the functional profile of the graft fibroblasts was defined before and after treatment with IgG from healthy controls or SSc patients. Two procedures were used to investigate the involvement of platelet\u2010derived growth factor receptor (PDGFR): 1) nilotinib, a tyrosine kinase inhibitor, was administered to mice before injection of IgG from SSc patient sera (SSc IgG) into the grafts, and 2) human anti\u2010PDGFR monoclonal antibodies were injected into the grafts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Depending on the type of bioengineered skin grafted, the regenerated human skin exhibited either the typical scleroderma phenotype or the healthy human skin architecture. Treatment of animals carrying healthy donor skin grafts with SSc IgG resulted in the appearance of a bona fide scleroderma phenotype, as confirmed by increased collagen deposition and fibroblast activation markers. Results of the experiments involving administration of nilotinib or monoclonal antibodies confirmed the involvement of PDGFR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results provide the first in vivo demonstration of the fibrotic properties of anti\u2010PDGFR agonistic antibodies. This bioengineered skin\u2013humanized mouse model can be used to test in vivo the progression of the disease and to monitor response to antifibrotic drugs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.39728","type":"journal-article","created":{"date-parts":[[2016,4,25]],"date-time":"2016-04-25T20:22:49Z","timestamp":1461615769000},"page":"2263-2273","source":"Crossref","is-referenced-by-count":45,"title":["EXPRESSION OF CONCERN: Induction of Scleroderma Fibrosis in Skin\u2010Humanized Mice by Administration of Anti\u2212Platelet\u2010Derived Growth Factor Receptor Agonistic Autoantibodies"],"prefix":"10.1002","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9132-7401","authenticated-orcid":false,"given":"Michele M.","family":"Luchetti","sequence":"first","affiliation":[{"name":"Universit\u00e0 Politecnica delle Marche Ancona 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