{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T05:19:44Z","timestamp":1769059184884,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Raynaud's and Scleroderma Association"},{"name":"Bath Institute for Rheumatic Diseases"},{"name":"Asmarley Charitable Trust"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate novel systemic sclerosis (SSc) autoantibodies in autoantibody\u2010negative patients and establish clinical associations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum samples and clinical data for 548 patients with SSc were collected. Routine serologic techniques were used to test the serum samples for known SSc autoantibodies, and samples with negative results were further investigated by radiolabeled\u2010protein immunoprecipitation assay. Sera that immunoprecipitated a novel 30\u2010kd band were analyzed by indirect immunofluorescence and immunoprecipitation, using depleted cell extracts to establish a common reactivity. Mass spectrometry was performed to identify the novel autoantigen, and the results were confirmed using commercial antibodies. Sera from 426 patients with other forms of connective tissue disease, 103 with rheumatoid arthritis, 114 with idiopathic interstitial lung disease (ILD), and 150 healthy subjects were serotyped as controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A novel autoantigen with a molecular weight of \u223c30 kd was recognized by 7 sera from patients with SSc, 6 of whom had ILD, and by no controls. Six of the patients had diffuse cutaneous involvement, and 4 had overlap features with other autoimmune diseases. Immunodepletion experiments indicated that all samples targeted the same autoantigen, and mass spectrometry identified the novel autoantigen as eukaryotic initiation factor 2B (eIF2B).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We report the identification of a novel autoantibody (anti\u2010eIF2B) in a small number of patients with SSc (\u223c1%); this autoantibody is closely associated with diffuse cutaneous manifestations and the presence of ILD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.39755","type":"journal-article","created":{"date-parts":[[2016,6,6]],"date-time":"2016-06-06T09:01:58Z","timestamp":1465203718000},"page":"2778-2783","source":"Crossref","is-referenced-by-count":39,"title":["Brief Report: Anti\u2013Eukaryotic Initiation Factor 2B Autoantibodies Are Associated With Interstitial Lung Disease in Patients With Systemic Sclerosis"],"prefix":"10.1002","volume":"68","author":[{"given":"Zoe E.","family":"Betteridge","sequence":"first","affiliation":[{"name":"University of Bath Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Woodhead","sequence":"additional","affiliation":[{"name":"Glenfield Hospital Leicester UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Lu","sequence":"additional","affiliation":[{"name":"University of Bath Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gavin","family":"Shaddick","sequence":"additional","affiliation":[{"name":"University of Bath Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher C.","family":"Bunn","sequence":"additional","affiliation":[{"name":"Royal Free Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher P.","family":"Denton","sequence":"additional","affiliation":[{"name":"Royal Free Hospital and University College London Medical School London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Abraham","sequence":"additional","affiliation":[{"name":"University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland M.","family":"du Bois","sequence":"additional","affiliation":[{"name":"Imperial College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mervyn","family":"Lewis","sequence":"additional","affiliation":[{"name":"University of the West of England Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athol U.","family":"Wells","sequence":"additional","affiliation":[{"name":"Royal Brompton Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil J.","family":"McHugh","sequence":"additional","affiliation":[{"name":"University of Bath Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-857X(03)00015-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.05.011"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rdc.2007.12.001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000113"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230510"},{"key":"e_1_2_7_7_1","first-page":"202","article-title":"Scleroderma (systemic sclerosis): classification, subsets and pathogenesis","volume":"15","author":"LeRoy EC","year":"1988","journal-title":"J Rheumatol"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.41.5.554"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10760"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0331487"},{"issue":"3","key":"e_1_2_7_11_1","first-page":"167","article-title":"Identification of autoantibodies to a novel autoantigen protein complex (EIF3) in polymyositis patients","volume":"50","author":"Betteridge Z","year":"2011","journal-title":"Rheumatology (Oxford)"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-5107-5_4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/08923973.2011.572262"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12200"}],"container-title":["Arthritis &amp; 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