{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:43:31Z","timestamp":1790808211470,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,10,9]],"date-time":"2016-10-09T00:00:00Z","timestamp":1475971200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Groupe Fran\u00e7ais de Recherch\u00e9 sur la Scl\u00e9rodermie"},{"name":"Soci\u00e9t\u00e9 Fran\u00e7aise de Rhumatologie"},{"name":"UCB (SIRIUS grant)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the relationship between vascular damage and fibrosis in systemic sclerosis (SSc) by testing the hypothesis that platelets contribute to skin fibrosis via the activation of human dermal microvascular endothelial cells (HDMECs) and subsequent production of profibrotic mediators.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 203 SSc patients and 30 healthy donors were prospectively enrolled between 2012 and 2015 at the University Hospital of Bordeaux. Immunohistochemistry and immunofluorescence analyses were performed on skin biopsy sections from 18 SSc patients and 5 healthy donors. Serum thymic stromal lymphopoietin (TSLP) levels were measured by enzyme\u2010linked immunosorbent assay in the entire cohort. HDMECs and fibroblasts were purified from biopsy sections. Extracellular matrix production by cultured fibroblasts was assessed by real\u2010time quantitative polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum TSLP levels were significantly increased in SSc patients compared to healthy donors (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001) and were associated with a higher frequency of vasculopathy (<jats:italic>P<\/jats:italic>\u2009=\u20090.02). The proportion of TSLP\u2010positive dermal cells was increased in the skin of SSc patients compared with healthy donors (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001) and was correlated with fibrosis (modified Rodnan skin thickness score) (r\u2009=\u20090.6146, <jats:italic>P<\/jats:italic>\u2009=\u20090.0001). In SSc dermis, TSLP was mainly expressed by CD31\u2010positive endothelial cells. In vitro, activated platelets induced TSLP production by HDMECs in an interleukin\u20101\u03b2\u2013dependent manner. SSc fibroblasts responded differently according to their original TSLP environment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken together, these results identify HDMECs as contributors to TSLP production in SSc and suggest a potential mechanism by which platelets may profoundly affect the fibrotic process in SSc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.39817","type":"journal-article","created":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T11:42:46Z","timestamp":1468842166000},"page":"2784-2794","source":"Crossref","is-referenced-by-count":49,"title":["Platelets Induce Thymic Stromal Lymphopoietin Production by Endothelial Cells: Contribution to Fibrosis in Human Systemic Sclerosis"],"prefix":"10.1002","volume":"68","author":[{"given":"Marie\u2010Elise","family":"Truchetet","sequence":"first","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University and Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Demoures","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Eduardo Guimaraes","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University, Bordeaux, France, and Federal University of Alagoas Macei\u00f3 Alagoas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Bertrand","sequence":"additional","affiliation":[{"name":"Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pa\u00f4line","family":"Laurent","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Jolivel","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Douchet","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ment","family":"Jacquemin","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane","family":"Khoryati","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Duffau","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University and Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estibaliz","family":"Lazaro","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University and Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Richez","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University and Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Seneschal","sequence":"additional","affiliation":[{"name":"Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Sylvie","family":"Doutre","sequence":"additional","affiliation":[{"name":"Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Pellegrin","sequence":"additional","affiliation":[{"name":"Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00ebl","family":"Constans","sequence":"additional","affiliation":[{"name":"Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Schaeverbeke","sequence":"additional","affiliation":[{"name":"Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Blanco","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University and Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Contin\u2010Bordes","sequence":"additional","affiliation":[{"name":"CNRS\u2010UMR 5164 Immuno ConcEpT, Bordeaux University and Bordeaux Hospital Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"on behalf of the F\u00e9d\u00e9ration Hospitalo\u2010Universitaire ACRONIM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0806188"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00272"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711660307"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-96-5-610"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3282eeb3a4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kep154"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2005.03.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2012.719946"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1114576"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101629"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kev241"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400864"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051135"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20102131"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300588"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.37859"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-202279"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38098"},{"key":"e_1_2_7_20_1","first-page":"1281","article-title":"Inter and intraobserver variability of total skin thickness score (modified Rodnan TSS) in systemic sclerosis","volume":"22","author":"Clements P","year":"1995","journal-title":"J Rheumatol"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.37860"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4334"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911726107"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001806"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.1.134"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI77958"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008264"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2807"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12727"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni805"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1613"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1247"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.118"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00177-8"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001001"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2004.022970"}],"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.39817","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.39817","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.39817","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:32:42Z","timestamp":1759948362000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.39817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,9]]},"references-count":35,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/art.39817"],"URL":"https:\/\/doi.org\/10.1002\/art.39817","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,9]]}}}