{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:29:48Z","timestamp":1790994588165,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,4,26]],"date-time":"2017-04-26T00:00:00Z","timestamp":1493164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"LabEx Inflamex, Universit\u00e9 Paris-Diderot, Paris, France","award":["ANR-11-IDEX-0005-02"],"award-info":[{"award-number":["ANR-11-IDEX-0005-02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the role of B lymphocytes in systemic sclerosis (SSc).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral B cell subpopulations and the production of interleukin\u20106 (IL\u20106) and transforming growth factor \u03b2 (TGF\u03b2) were analyzed using flow cytometry and multiplex assay. The fibroblast proliferation rate upon incubation with supernatants from B cells isolated from SSc patients or healthy controls was assessed using XTT, bromodeoxyuridine, and Ki\u201067. Collagen production was assessed using a collagen assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ninety untreated patients (12 males) fulfilling the American College of Rheumatology\/European League Against Rheumatism criteria for SSc (23 with diffuse cutaneous SSc [dcSSc] and 67 with limited cutaneous SSc [lcSSc]) and 30 healthy controls were recruited. Increased proportions of B cells expressing CD69 and CD95 were identified among the patients with SSc. B lymphocytes from dcSSc patients versus lcSSc patients and healthy controls expressed increased proportion of cells positive for CD5 (mean\u2009\u00b1\u2009SD 24.12\u2009\u00b1\u20097.93% versus 14.09\u2009\u00b1\u20096.58% [<jats:italic>P<\/jats:italic>\u2009=\u20090.03] and 14.21\u2009\u00b1\u20095.34% [<jats:italic>P<\/jats:italic>\u2009=\u20090.01]), CD86 (39.89\u2009\u00b1\u200922.11% versus 17.72\u2009\u00b1\u200913.98% [<jats:italic>P<\/jats:italic>\u2009=\u20090.0007] and 11.68\u2009\u00b1\u200911.09% [<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001]), IL\u20106 receptor (IL\u20106R; 33.64\u2009\u00b1\u200923.12% versus 17.91\u2009\u00b1\u200913.62% [<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001] and 12.08\u2009\u00b1\u20098.68% [<jats:italic>P<\/jats:italic>\u2009=\u20090.0009]), or IL\u201021R (32.55\u2009\u00b1\u200920.19% versus 5.76\u2009\u00b1\u20094.40% [<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001] and 5.93\u2009\u00b1\u20093.29% [<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001]). In addition, the levels of IL\u20106 (mean\u2009\u00b1\u2009SD 314.3\u2009\u00b1\u2009317.8 pg\/ml versus 6.10\u2009\u00b1\u20092.58 pg\/ml; <jats:italic>P<\/jats:italic>\u2009=\u20090.0007) and TGF\u03b2 (mean\u2009\u00b1\u2009SD 1,020\u2009\u00b1\u2009569 pg\/ml versus 163.8\u2009\u00b1\u200998.69 pg\/ml; <jats:italic>P<\/jats:italic>\u2009=\u20090.001) secreted by B lymphocytes from patients with SSc were increased compared to healthy controls. Fibroblast proliferation and collagen production were also significantly increased in the presence of B cell supernatant from SSc patients as compared to healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The numbers of activated B cells were increased in SSc patients, and the up\u2010regulation of CD5, CD86, IL\u20106R, and IL\u201021R discriminated between patients with dcSSc and those with lcSSc. Peripheral B lymphocytes from SSc patients secreted both IL\u20106 and TGF\u03b2, and they activated fibroblasts in vitro.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.40016","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T16:13:48Z","timestamp":1482164028000},"page":"1078-1089","source":"Crossref","is-referenced-by-count":88,"title":["Scleroderma Peripheral B Lymphocytes Secrete Interleukin\u20106 and Transforming Growth Factor \u03b2 and Activate Fibroblasts"],"prefix":"10.1002","volume":"69","author":[{"given":"Nicolas","family":"Dumoitier","sequence":"first","affiliation":[{"name":"INSERM U1016, Institut Cochin, CNRS UMR 8104, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, LabEx Inflamex, Universit\u00e9 Sorbonne Paris Cit\u00e9 and Universit\u00e9 Paris Diderot Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Chaigne","sequence":"additional","affiliation":[{"name":"INSERM U1016, Institut Cochin, CNRS UMR 8104, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9 and Service de M\u00e9decine Interne, Centre de R\u00e9f\u00e9rence Maladies Syst\u00e9miques Autoimmunes Rares, Vascularites N\u00e9crosantes et Scl\u00e9rodermie Syst\u00e9mique, H\u00f4pital Cochin, AP\u2010HP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"R\u00e9gent","sequence":"additional","affiliation":[{"name":"INSERM U1016, Institut Cochin, CNRS UMR 8104, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9 and Service de M\u00e9decine Interne, Centre de R\u00e9f\u00e9rence Maladies Syst\u00e9miques Autoimmunes Rares, Vascularites N\u00e9crosantes et Scl\u00e9rodermie Syst\u00e9mique, H\u00f4pital Cochin, AP\u2010HP Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Lofek","sequence":"additional","affiliation":[{"name":"INSERM U1016, Institut Cochin, CNRS UMR 8104 and Universit\u00e9 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