{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:51:27Z","timestamp":1789717887907,"version":"4.0.1"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T00:00:00Z","timestamp":1510185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K09893"],"award-info":[{"award-number":["16K09893"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis & Rheumatology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Objective<\/jats:title>SKG<\/jats:styled-content> mice develop interstitial lung disease (ILD<\/jats:styled-content>) resembling rheumatoid arthritis\u2013associated ILD<\/jats:styled-content> in humans. The aim of this study was to clarify the mechanism underlying the lung pathology by analyzing lung\u2010infiltrating cells in SKG<\/jats:styled-content> mice with ILD<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>We assessed the severity of zymosan A (ZyA)\u2013induced ILD<\/jats:styled-content> in SKG<\/jats:styled-content> mice histologically, and we examined lung\u2010infiltrating cells by flow cytometry. Total lung cells and isolated monocytic myeloid\u2010derived suppressor cells (MDSC<\/jats:styled-content>s) were cultured in\u00a0vitro with granulocyte\u2013macrophage colony\u2010stimulating factor (GM<\/jats:styled-content>\u2010CSF<\/jats:styled-content>) and interleukin\u20104. The proliferation of 5,6\u2010carboxyfluorescein diacetate N<\/jats:italic>\u2010succinimidyl ester\u2013labeled naive T cells cocultured with isolated CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> cells and MDSC<\/jats:styled-content>s was evaluated by flow cytometry. CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> cells were adoptively transferred to ZyA\u2010treated SKG<\/jats:styled-content> mice.<\/jats:p><\/jats:sec>Results<\/jats:title>MDSC<\/jats:styled-content>s, Th17 cells, and group 1 and 3 innate lymphoid cells (ILC<\/jats:styled-content>1s and ILC<\/jats:styled-content>3s) were increased in the lungs; the proportion of these cells varied with ILD<\/jats:styled-content> severity. In this process, we found that a unique cell population, CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> cells, was expanded in the severely inflamed lungs. Approximately half of the CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> cells expressed CD<\/jats:styled-content>11c. CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> cells were induced from monocytic MDSC<\/jats:styled-content>s with GM<\/jats:styled-content>\u2010CSF<\/jats:styled-content> in\u00a0vitro and were considered tolerogenic because they suppressed T cell proliferation. These CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> cells have never been described previously, and we termed them CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> tolerogenic dendritic cell (DC<\/jats:styled-content>)\u2013like cells. Th17 cells, ILC<\/jats:styled-content>1s, and ILC<\/jats:styled-content>3s in the inflamed lung produced GM<\/jats:styled-content>\u2010CSF<\/jats:styled-content>, which may have expanded CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> tolerogenic DC<\/jats:styled-content>\u2013like cells in\u00a0vivo. Furthermore, adoptive transfer of CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> tolerogenic DC<\/jats:styled-content>\u2013like cells significantly suppressed progression of ILD<\/jats:styled-content> in SKG<\/jats:styled-content> mice.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We identified unique suppressive myeloid cells that were differentiated from monocytic MDSC<\/jats:styled-content>s in SKG<\/jats:styled-content> mice with ILD<\/jats:styled-content>, and we termed them CD<\/jats:styled-content>11b+Gr\u20101dim<\/jats:sup> tolerogenic DC<\/jats:styled-content>\u2013like cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.40231","type":"journal-article","created":{"date-parts":[[2017,8,13]],"date-time":"2017-08-13T22:12:35Z","timestamp":1502662355000},"page":"2314-2327","source":"Crossref","is-referenced-by-count":23,"title":["CD<\/scp>11b+Gr\u20101dim<\/sup> Tolerogenic Dendritic Cell\u2013Like Cells Are Expanded in Interstitial Lung Disease in SKG<\/scp> Mice"],"prefix":"10.1002","volume":"69","author":[{"given":"Sho","family":"Sendo","sequence":"first","affiliation":[{"name":"Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Saegusa","sequence":"additional","affiliation":[{"name":"Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaichi","family":"Okano","sequence":"additional","affiliation":[{"name":"Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kengo","family":"Akashi","sequence":"additional","affiliation":[{"name":"Kobe University Graduate School of Medicine Kobe 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