{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T21:31:48Z","timestamp":1774128708354,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T00:00:00Z","timestamp":1535932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K23 AR064279"],"award-info":[{"award-number":["K23 AR064279"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30\u2010AR\u2010053503"],"award-info":[{"award-number":["P30\u2010AR\u2010053503"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30\u2010AR\u2010070254"],"award-info":[{"award-number":["P30\u2010AR\u2010070254"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 DE12354"],"award-info":[{"award-number":["R01 DE12354"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R37NS041435"],"award-info":[{"award-number":["R37NS041435"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005984","name":"Dr. Miriam and Sheldon G. Adelson Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005984","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Autoantibodies are clinically useful for phenotyping patients across the spectrum of autoimmune rheumatic diseases. Using serum from a patient with Sj\u00f6gren's syndrome (<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>), we detected a new specificity by immunoblotting. This study was undertaken to identify this autoantibody and to evaluate its disease specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prominent 40\u2010kd band was detected when immunoblotting was performed using <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> patient serum and lysate from rat dorsal root ganglia (<jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content>s). Using 2\u2010dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry peptide sequencing, the autoantigen was identified as calponin 3. Anti\u2013calponin 3 antibodies were evaluated in sera from patients with primary <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> (n = 209), patients with systemic lupus erythematosus (<jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content>; n = 138), patients with myositis (n = 138), patients with multiple sclerosis (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>; n = 44), and healthy controls (n = 46) by enzyme\u2010linked immunosorbent assay. Expression of calponin 3 was assessed by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Calponin 3 was identified as a new autoantigen. Anti\u2013calponin 3 antibodies were detected in 23 (11.0%) of the 209 <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> patients, 12 (8.7%) of the 138 <jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content> patients, 7 (5.1%) of the 138 myositis patients, 3 (6.8%) of the 44 <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> patients, and 1 (2.2%) of the 46 healthy controls. Among <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> patients, the frequency of anti\u2013calponin 3 antibodies was highest in those with neuropathies (7 [17.9%] of 39). In this subset, the frequency of anti\u2013calponin 3 antibodies differed significantly from that in the control group (<jats:italic>P<\/jats:italic> = 0.02). Calponin 3 was expressed primarily in rat <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> perineuronal satellite cells but not neurons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Calponin 3 is a novel autoantigen. Antibodies against this protein are found in <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content> and associate with the subset of patients experiencing neuropathies. Intriguingly, we found that calponin 3 is expressed in <jats:styled-content style=\"fixed-case\">DRG<\/jats:styled-content> perineuronal satellite cells, suggesting that these may be a target in <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.40550","type":"journal-article","created":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T05:04:27Z","timestamp":1526015067000},"page":"1610-1616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Brief Report: Anti\u2013Calponin 3 Autoantibodies: A Newly Identified Specificity in Patients With Sj\u00f6gren\u2019s Syndrome"],"prefix":"10.1002","volume":"70","author":[{"given":"Julius","family":"Birnbaum","sequence":"first","affiliation":[{"name":"Johns Hopkins University Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Hoke","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aliya","family":"Lalji","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Calabresi","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavan","family":"Bhargava","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia","family":"Casciola\u2010Rosen","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,9,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.17718"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1998.0265"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/onci.27384"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.6.554"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.21591"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2004.11.007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/NRL.0b013e3181ebe59f"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.55.12.1513"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000000005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.22366"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8027.2007.00131.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.07.024"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.02.040"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410270313"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22541"}],"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.40550","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.40550","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:44:33Z","timestamp":1759949073000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.40550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,3]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1002\/art.40550"],"URL":"https:\/\/doi.org\/10.1002\/art.40550","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,3]]},"assertion":[{"value":"2017-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}