{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T21:59:17Z","timestamp":1793397557122,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,29]],"date-time":"2019-01-29T00:00:00Z","timestamp":1548720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To elucidate the role of the fractalkine (FKN)\/CX<jats:sub>3<\/jats:sub>CR1 pathway in joint destruction in rheumatoid arthritis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the effect of treatment with anti\u2013mouse FKN (anti\u2010mFKN) monoclonal antibody (mAb) on joint destruction and the migration of osteoclast precursors (OCPs) into the joint, using the collagen\u2010induced arthritis (CIA) model.<\/jats:p><jats:p>DBA\/1 mice were immunized with bovine type II collagen to induce arthritis, and then treated with anti\u2010mFKN mAb. Disease severity was monitored by arthritis score, and joint destruction was evaluated by soft x\u2010ray and histologic analyses. Plasma levels of joint destruction markers were assessed by enzyme\u2010linked immunosorbent assay. FKN expression on endothelial cells was detected by immunohistochemistry. Bone marrow\u2013derived OCPs were labeled with fluorescein and transferred to mice with CIA, and the migration of the OCPs to the joints was then analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both prophylactic and therapeutic treatment with anti\u2010mFKN mAb significantly decreased the arthritis and soft x\u2010ray scores. Plasma levels of cartilage oligomeric matrix protein and matrix metalloproteinase 3 decreased after treatment with anti\u2010mFKN mAb. Histologic analysis revealed that anti\u2010mFKN mAb inhibited synovitis, pannus formation, and cartilage destruction, as well as suppressed bone damage, with a marked reduction in the number of tartrate\u2010resistant acid phosphatase\u2013positive osteoclasts. Anti\u2010mFKN mAb strongly inhibited the migration of bone marrow\u2013derived OCPs into the affected synovium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2010mFKN mAb notably ameliorates arthritis and joint destruction in the CIA model, as well as inhibits migration of OCPs into the synovium. These results suggest that inhibition of the FKN\/CX<jats:sub>3<\/jats:sub>CR1 pathway could be a novel strategy for treatment of both synovitis and joint destruction in rheumatoid arthritis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.40688","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T05:49:43Z","timestamp":1533620983000},"page":"222-231","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Role of Anti\u2010Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis"],"prefix":"10.1002","volume":"71","author":[{"given":"Kana","family":"Hoshino\u2010Negishi","sequence":"first","affiliation":[{"name":"KAN Research Institute, Inc.  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