{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:46:31Z","timestamp":1791899191638,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T00:00:00Z","timestamp":1559779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1047252"],"award-info":[{"award-number":["1047252"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1059167"],"award-info":[{"award-number":["1059167"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["AR049610"],"award-info":[{"award-number":["AR049610"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Arthritogenic alphaviruses, such as Ross River virus (<jats:styled-content style=\"fixed-case\">RRV<\/jats:styled-content>) and chikungunya virus (<jats:styled-content style=\"fixed-case\">CHIKV<\/jats:styled-content>), particularly affect joints of the extremities and can lead to debilitating and potentially chronic polyarthritis\/polyarthralgia. The innate immune response of the host plays a crucial role in inducing proinflammatory host factors, leading to tissue destruction and bone loss in the joints. This study was performed to assess how the inhibition of interleukin\u20101\u03b2 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2) signaling using the clinical rheumatoid arthritis drug anakinra influences bone loss in mice with arthritogenic alphavirus infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice (n = 5 per group) were infected with <jats:styled-content style=\"fixed-case\">RRV<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">CHIKV<\/jats:styled-content> and then treated with anakinra. Weight gain and disease severity were measured, tissue viral titers were determined, and histologic changes in joint tissues were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anakinra therapy reduced <jats:styled-content style=\"fixed-case\">RRV<\/jats:styled-content>\u2010 and <jats:styled-content style=\"fixed-case\">CHIKV<\/jats:styled-content>\u2010induced bone loss in this murine model (<jats:italic>P<\/jats:italic> &lt; 0.001 and <jats:italic>P<\/jats:italic> &lt; 0.05, respectively). Histologic analysis of the knee joint showed that treatment with anakinra decreased epiphyseal growth plate thinning, loss of epiphyseal bone volume, and osteoclastogenesis in the tibia. Importantly, pharmacologic <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101 receptor (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101R) blockade did not improve other clinical features, including disease score, weight loss, or viremia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present findings suggest that anakinra therapy may reduce bone loss in experimental murine models of <jats:styled-content style=\"fixed-case\">RRV<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CHIKV<\/jats:styled-content>. Further investigations are needed to assess the potential therapeutic benefits of anakinra in patients with arthritogenic alphavirus disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.40856","type":"journal-article","created":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T07:21:11Z","timestamp":1549956071000},"page":"1185-1190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Inhibition of Interleukin\u20101\u03b2 Signaling by Anakinra Demonstrates a Critical Role of Bone Loss in Experimental Arthritogenic Alphavirus Infections"],"prefix":"10.1002","volume":"71","author":[{"given":"Stefan","family":"Wolf","sequence":"first","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Taylor","sequence":"additional","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2528-2783","authenticated-orcid":false,"given":"Ali","family":"Zaid","sequence":"additional","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Freitas","sequence":"additional","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5661-9828","authenticated-orcid":false,"given":"Lara J.","family":"Herrero","sequence":"additional","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shambhavi","family":"Rao","sequence":"additional","affiliation":[{"name":"National Institute of Virology  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Suhrbier","sequence":"additional","affiliation":[{"name":"QIMR Berghofer Medical Research Institute University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark R.","family":"Forwood","sequence":"additional","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Bucala","sequence":"additional","affiliation":[{"name":"Yale University School of Medicine  New Haven, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3141-8410","authenticated-orcid":false,"given":"Suresh","family":"Mahalingam","sequence":"additional","affiliation":[{"name":"Griffith University  Gold Coast Campus Southport Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30385-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2002.tb04837.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900255"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/acr.22900"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1753-6405.12515"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiq042"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv049"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2010.01.011"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1318859111"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1002\/art.38090","article-title":"Macrophage migration inhibitory factor receptor CD74 mediates alphavirus\u2010induced arthritis and myositis in murine models of alphavirus infection","volume":"65","author":"Herrero L","year":"2013","journal-title":"Arthritis Rheum"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1586\/1744666X.2.3.331"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/35.12.3680930"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30112"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.10.6441-6448.2005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-017-0015-4"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1128\/JVI.73.3.2563-2567.1999","article-title":"Resistance of interleukin\u20101\u03b2\u2010deficient mice to fatal Sindbis virus encephalitis","volume":"73","author":"Liang XH","year":"1999","journal-title":"J Virol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-217"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24682"}],"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.40856","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.40856","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/art.40856","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.40856","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:51:06Z","timestamp":1759945866000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.40856"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,6]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1002\/art.40856"],"URL":"https:\/\/doi.org\/10.1002\/art.40856","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,6,6]]},"assertion":[{"value":"2017-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}