{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T19:40:37Z","timestamp":1793907637510,"version":"4.8.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis & Rheumatology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"Objective<\/jats:title>Genetic associations imply a role for CD<\/jats:styled-content>8+ T cells and the interleukin\u201023 (IL<\/jats:styled-content>\u201023)\/IL<\/jats:styled-content>\u201017 axis in psoriatic arthritis (PsA) and other spondyloarthritides (SpA). IL<\/jats:styled-content>\u201017A+CD<\/jats:styled-content>8+ (Tc17) T cells are enriched in the synovial fluid (SF<\/jats:styled-content>) of patients with PsA, and IL<\/jats:styled-content>\u201017A blockade is clinically efficacious in PsA\/SpA. This study was undertaken to determine the immunophenotype, molecular profile, and function of synovial Tc17 cells in order to elucidate their role in PsA\/SpA pathogenesis.<\/jats:p><\/jats:sec>Methods<\/jats:title>Peripheral blood (PB<\/jats:styled-content>) and SF<\/jats:styled-content> mononuclear cells were isolated from patients with PsA or other types of SpA. Cells were phenotypically, transcriptionally, and functionally analyzed by flow cytometry (n = 6\u201318), T cell receptor \u03b2 (TCR<\/jats:styled-content>\u03b2) sequencing (n = 3), RNA<\/jats:styled-content>\u2010Seq (n = 3), quantitative reverse transcriptase\u2013polymerase chain reaction (n = 4), and Luminex or enzyme\u2010linked immunosorbent assay (n = 4\u201316).<\/jats:p><\/jats:sec>Results<\/jats:title>IL<\/jats:styled-content>\u201017A+CD<\/jats:styled-content>8+ T cells were predominantly TCR<\/jats:styled-content>\u03b1\u03b2+ and their frequencies were increased in the SF<\/jats:styled-content> versus the PB<\/jats:styled-content> of patients with established PsA (P<\/jats:italic> < 0.0001) or other SpA (P<\/jats:italic> = 0.0009). TCR<\/jats:styled-content>\u03b2 sequencing showed that these cells were polyclonal in PsA (median clonality 0.08), while RNA<\/jats:styled-content>\u2010Seq and deep immunophenotyping revealed that PsA synovial Tc17 cells had hallmarks of Th17 cells (RORC<\/jats:styled-content><\/jats:italic>\/IL<\/jats:styled-content><\/jats:italic>23R\/CCR<\/jats:styled-content>6<\/jats:italic>\/CD<\/jats:styled-content>161<\/jats:italic>) and Tc1 cells (granzyme A\/B). Synovial Tc17 cells showed a strong tissue\u2010resident memory T (Trm) cell signature and secreted a range of proinflammatory cytokines. We identified CXCR<\/jats:styled-content>6 as a marker for synovial Tc17 cells, and increased levels of CXCR<\/jats:styled-content>6 ligand CXCL<\/jats:styled-content>16 in PsA SF<\/jats:styled-content> (P<\/jats:italic> = 0.0005), which may contribute to their retention in the joint.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Our results identify synovial Tc17 cells as a polyclonal subset of Trm cells characterized by polyfunctional, proinflammatory mediator production and CXCR<\/jats:styled-content>6 expression. The molecular signature and functional profiling of these cells may help explain how Tc17 cells can contribute to synovial inflammation and disease persistence in PsA and possibly other types of SpA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.41156","type":"journal-article","created":{"date-parts":[[2019,11,2]],"date-time":"2019-11-02T10:24:41Z","timestamp":1572690281000},"page":"435-447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":124,"title":["Polyfunctional, Proinflammatory, Tissue\u2010Resident Memory Phenotype and Function of Synovial Interleukin\u201017A+CD<\/scp>8+ T Cells in Psoriatic Arthritis"],"prefix":"10.1002","volume":"72","author":[{"given":"Kathryn J. 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UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine D.","family":"Hughes","sequence":"additional","affiliation":[{"name":"King\u2019s College London Guy\u2019s Hospital, and St. Thomas\u2019 Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estee","family":"Chan","sequence":"additional","affiliation":[{"name":"Guy\u2019s Hospital and St. Thomas\u2019 Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce W.","family":"Kirkham","sequence":"additional","affiliation":[{"name":"Guy\u2019s Hospital and St. Thomas\u2019 Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9337-7194","authenticated-orcid":false,"given":"Leonie S.","family":"Taams","sequence":"additional","affiliation":[{"name":"King\u2019s College London London 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