{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:40:10Z","timestamp":1792633210234,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001922","name":"Efficacy and Mechanism Evaluation Programme","doi-asserted-by":"publisher","award":["11\/100\/76"],"award-info":[{"award-number":["11\/100\/76"]}],"id":[{"id":"10.13039\/501100001922","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["36661"],"award-info":[{"award-number":["36661"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/K015346\/1"],"award-info":[{"award-number":["MR\/K015346\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis & Rheumatology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"\n Objective<\/jats:title>\n \n To define the relationship of synovial B cells to clinical phenotypes at different stages of disease evolution and drug exposure in rheumatoid arthritis (\n RA<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Synovial biopsy specimens and demographic and clinical data were collected from 2\n RA<\/jats:styled-content>\n cohorts (n = 329), one of patients with untreated early\n RA<\/jats:styled-content>\n (n = 165) and one of patients with established\n RA<\/jats:styled-content>\n with an inadequate response to tumor necrosis factor inhibitors (\n TNF<\/jats:styled-content>\n i\u2010\n IR<\/jats:styled-content>\n ; n = 164). Synovial tissue was subjected to hematoxylin and eosin and immunohistochemical staining and semiquantitative assessment for the degree of synovitis (on a scale of 0\u20139) and of\n CD<\/jats:styled-content>\n 20+ B cell infiltrate (on a scale of 0\u20134). B cell scores were validated by digital image analysis and B cell lineage\u2013specific transcript analysis (\n RNA<\/jats:styled-content>\n \u2010Seq) in the early\n RA<\/jats:styled-content>\n (n = 91) and\n TNF<\/jats:styled-content>\n i\u2010\n IR<\/jats:styled-content>\n (n = 127) cohorts. Semiquantitative\n CD<\/jats:styled-content>\n 20 scores were used to classify patients as B cell rich (\u22652) or B cell poor (<2).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Semiquantitative B cell scores correlated with digital image analysis quantitative measurements and B cell lineage\u2013specific transcripts. B cell\u2013rich synovitis was present in 35% of patients in the early\n RA<\/jats:styled-content>\n cohort and 47.7% of patients in the\n TNF<\/jats:styled-content>\n i\u2010\n IR<\/jats:styled-content>\n cohort (\n P<\/jats:italic>\n = 0.025). B cell\u2013rich patients showed higher levels of disease activity and seropositivity for rheumatoid factor and anti\u2013citrullinated protein antibody in early\n RA<\/jats:styled-content>\n but not in established\n RA<\/jats:styled-content>\n , while significantly higher histologic synovitis scores in B cell\u2013rich patients were demonstrated in both cohorts.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n We describe a robust semiquantitative histologic B cell score that closely replicates the quantification of B cells by digital or molecular analyses. Our findings indicate an ongoing B cell\u2013rich synovitis, which does not seem to be captured by standard clinimetric assessment, in a larger proportion of patients with established\n RA<\/jats:styled-content>\n than early\n RA<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/art.41184","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T05:01:50Z","timestamp":1575262910000},"page":"714-725","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["B Cell Synovitis and Clinical Phenotypes in Rheumatoid Arthritis: Relationship to Disease Stages and Drug Exposure"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6759-7521","authenticated-orcid":false,"given":"F.","family":"Rivellese","sequence":"first","affiliation":[{"name":"Barts and The London School of Medicine and Dentistry Queen Mary University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1280-9133","authenticated-orcid":false,"given":"F.","family":"Humby","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine and Dentistry Queen Mary University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Bugatti","sequence":"additional","affiliation":[{"name":"IRCCS Policlinico San Matteo Foundation and University of Pavia Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3757-3999","authenticated-orcid":false,"given":"L.","family":"Fossati\u2010Jimack","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine and Dentistry Queen Mary University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Rizvi","sequence":"additional","affiliation":[{"name":"Barts Health NHS Trust London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8019-556X","authenticated-orcid":false,"given":"D.","family":"Lucchesi","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine and Dentistry Queen Mary University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Lliso\u2010Ribera","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine and Dentistry Queen Mary University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Nerviani","sequence":"additional","affiliation":[{"name":"Barts and The London School of Medicine and Dentistry Queen Mary University of London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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