{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T04:00:20Z","timestamp":1774065620970,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004243","name":"Prinses Beatrix Spierfonds","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009248","name":"Innovatiefonds Zorgverzekeraars","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009248","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess anti\u2013cytosolic 5\u2032\u2010nucleotidase 1A (anti\u2013cN\u20101A) autoantibodies in children with juvenile dermatomyositis (DM) and healthy controls, using 3 different methods of antibody detection, as well as verification of the results in an independent cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Anti\u2013cN\u20101A reactivity was assessed in 34 Dutch juvenile DM patients and 20 healthy juvenile controls using the following methods: a commercially available full\u2010length cN\u20101A enzyme\u2010linked immunosorbent assay (ELISA), a synthetic peptide ELISA, and immunoblotting with a lysate from cN\u20101A\u2013expressing HEK 293 cells. Sera from juvenile DM patients with active disease and those with disease in remission were analyzed. An independent British cohort of 110 juvenile DM patients and 43 healthy juvenile controls was assessed using an in\u2010house full\u2010length cN\u20101A ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anti\u2013cN\u20101A reactivity was not present in sera from juvenile DM patients or healthy controls when tested with the commercially available full\u2010length cN\u20101A ELISA or by immunoblotting, in either active disease or disease in remission. Additionally, in the British juvenile DM cohort, anti\u2013cN\u20101A reactivity was not detected. Three Dutch juvenile DM patients had weakly positive results for 1 of 3 synthetic cN\u20101A peptides measured by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Juvenile DM patients and young healthy individuals did not show anti\u2013cN\u20101A reactivity as assessed by different antibody detection techniques.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.41660","type":"journal-article","created":{"date-parts":[[2021,1,26]],"date-time":"2021-01-26T09:55:29Z","timestamp":1611654929000},"page":"1329-1333","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti\u2013Cytosolic 5\u2032\u2010Nucleotidase 1A Autoantibodies Are Absent in Juvenile Dermatomyositis"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0242-4275","authenticated-orcid":false,"given":"Anke","family":"Rietveld","sequence":"first","affiliation":[{"name":"Donders Institute for Brain, 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Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Schlumberger","sequence":"additional","affiliation":[{"name":"Institute for Experimental Immunology Euroimmun  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baziel","family":"van Engelen","sequence":"additional","affiliation":[{"name":"Donders Institute for Brain, Cognition, and Behaviour Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annet","family":"van Royen\u2010Kerkhof","sequence":"additional","affiliation":[{"name":"Wilhelmina Children\u2019s Hospital University Medical Center Utrecht, and Utrecht University  Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Lu","sequence":"additional","affiliation":[{"name":"University of Bath  Bath UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7495-1429","authenticated-orcid":false,"given":"Lucy","family":"Wedderburn","sequence":"additional","affiliation":[{"name":"NIHR Great Ormond Street Hospital Biomedical Research Centre Centre for Adolescent Rheumatology Versus Arthritis University College London Great Ormond Street Institute of Child Health  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiaan","family":"Saris","sequence":"additional","affiliation":[{"name":"Donders Institute for Brain, Cognition, and Behaviour Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Tansley","sequence":"additional","affiliation":[{"name":"University of Bath  Bath 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