{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:52:02Z","timestamp":1787500322808,"version":"build-2736575974"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI049954"],"award-info":[{"award-number":["AI049954"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["AR068974"],"award-info":[{"award-number":["AR068974"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Autoimmune diseases affect women disproportionately more than men. Estrogen is implicated in immune cell dysfunction, yet its precise molecular roles are not fully known. We recently identified new roles for serine\/arginine\u2010rich splicing factor 1 (SRSF1) in T cell function and autoimmunity. SRSF1 levels are decreased in T cells from patients with systemic lupus erythematosus (SLE) and are associated with active disease and comorbidity. However, the molecular mechanisms that control SRSF1 expression are unknown.\n                      <jats:italic>Srsf1<\/jats:italic>\n                      messenger RNA (mRNA) has a long 3\u2032\u2010untranslated region (3\u2032\u2010UTR), suggesting posttranscriptional control. This study was undertaken to investigate the role of estrogen and posttranscriptional mechanisms of SRSF1 regulation in T cells and SLE.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In silico bioinformatics analysis of\n                      <jats:italic>Srsf1<\/jats:italic>\n                      \u20133\u2032\u2010UTR revealed multiple microRNA (miRNA; miR)\u2013binding sites. Additional screening and literature searches narrowed down hsa\u2010miR\u201010b\u20105p for further study. Peripheral blood T cells from healthy individuals and SLE patients were evaluated for mRNA and miRNA expression by quantitative reverse transcription\u2013polymerase chain reaction, and SRSF1 protein levels were assessed by immunoblotting. T cells were cultured with \u03b2\u2010estradiol, and transient transfections were used to overexpress miRNAs. Luciferase assays were used to measure 3\u2032\u2010UTR activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We demonstrated that estrogen increased hsa\u2010miR\u201010b\u20105p expression in human T cells, and hsa\u2010miR\u201010b\u20105p down\u2010regulated SRSF1 protein expression. Mechanistically, hsa\u2010mir\u201010b\u20105p regulated SRSF1 posttranscriptionally via control of its 3\u2032\u2010UTR activity. Importantly, hsa\u2010miR\u201010b\u20105p expression levels were elevated in T cells from healthy women compared to healthy men and also elevated in T cells from SLE patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We identified a previously unrecognized molecular link between estrogen and gene regulation in immune cells, with potential relevance to systemic autoimmune disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.41787","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T07:30:02Z","timestamp":1620891002000},"page":"2052-2058","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Estrogen\u2010Induced hsa\u2010miR\u201010b\u20105p Is Elevated in T Cells From Patients With Systemic Lupus Erythematosus and Down\u2010Regulates Serine\/Arginine\u2010Rich Splicing Factor 1"],"prefix":"10.1002","volume":"73","author":[{"given":"Suruchi A.","family":"Ramanujan","sequence":"first","affiliation":[{"name":"Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, and Harvard College  Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena N.","family":"Cravens","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Krishfield","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7652-3826","authenticated-orcid":false,"given":"Vasileios C.","family":"Kyttaris","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6441-3264","authenticated-orcid":false,"given":"Vaishali R.","family":"Moulton","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Harvard Medical School  Boston Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02279"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40512"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1700770"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.01.035"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00611"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12061"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.091660"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214207110"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI127949"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keaa094"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40930"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.04.016"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/lupus-2018-000290"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2015.01.004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.05.006"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.01.009"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350606"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00578"}],"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.41787","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/art.41787","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.41787","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T12:34:38Z","timestamp":1759926878000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.41787"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,29]]},"references-count":18,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2021,11]]}},"alternative-id":["10.1002\/art.41787"],"URL":"https:\/\/doi.org\/10.1002\/art.41787","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,29]]},"assertion":[{"value":"2020-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}