{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:19:45Z","timestamp":1790961585375,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"am","delay-in-days":214,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T00:00:00Z","timestamp":1635724800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["ROI_AR063091_01A1"],"award-info":[{"award-number":["ROI_AR063091_01A1"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100003185","name":"National Psoriasis Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100003185","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006370","name":"Small Business Innovation Research","doi-asserted-by":"publisher","award":["1R43AR074363\u201001"],"award-info":[{"award-number":["1R43AR074363\u201001"]}],"id":[{"id":"10.13039\/100006370","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","award":["WI238753"],"award-info":[{"award-number":["WI238753"]}],"id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/R020515\/1"],"award-info":[{"award-number":["MR\/R020515\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the involvement of the CCR6\/CCL20 axis in psoriatic arthritis (PsA) and psoriasis (PsO) and to evaluate its potential as a therapeutic target.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>First, we quantified CCL20 levels in peripheral blood and synovial fluid from PsA patients and examined the presence of CCR6+ cells in synovial and tendon tissue. Utilizing an interleukin\u201023 minicircle DNA (IL\u201023 MC) mouse model exhibiting key features of both PsO and PsA, we investigated CCR6 and CCL20 expression as well as the preventive and therapeutic effect of CCL20 blockade. Healthy tendon stromal cells were stimulated in vitro with IL\u20101\u03b2 to assess the production of CCL20 by quantitative polymerase chain reaction and enzyme\u2010linked immunosorbent assay. The effect of conditioned media from stimulated tenocytes in inducing T cell migration was interrogated using a Transwell system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed an up\u2010regulation of both CCR6 and CCL20 in the enthesis of IL\u201023 MC\u2013treated mice, which was confirmed in human biopsy specimens. Specific targeting of the CCR6\/CCL20 axis with a CCL20 locked dimer (CCL20LD) blocked entheseal inflammation, leading to profound reductions in clinical and proinflammatory markers in the joints and skin of IL\u201023 MC\u2013treated mice. The stromal compartment in the tendon was the main source of CCL20 in this model and, accordingly, in vitro activated human tendon cells were able to produce this chemokine and to induce CCR6+ T cell migration, the latter of which could be blocked by CCL20LD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study highlights the pathogenic role of the CCR6\/CCL20 axis in enthesitis and introduces the prospect of a novel therapeutic approach for treating patients with PsO and PsA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.41882","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T17:54:13Z","timestamp":1622742853000},"page":"2271-2281","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Targeting the CCR6\/CCL20 Axis in Entheseal and Cutaneous Inflammation"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9416-1152","authenticated-orcid":false,"given":"Zhenrui","family":"Shi","sequence":"first","affiliation":[{"name":"University of California, Davis, Sacramento, and Sun Yat\u2010sen Memorial Hospital and Sun Yat\u2010sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Garcia\u2010Melchor","sequence":"additional","affiliation":[{"name":"University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuesong","family":"Wu","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0143-1561","authenticated-orcid":false,"given":"Anthony E.","family":"Getschman","sequence":"additional","affiliation":[{"name":"Medical College of Wisconsin  Milwaukee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mimi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas\u00a0J.","family":"Rowland","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Machelle","family":"Wilson","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"Sunzini","sequence":"additional","affiliation":[{"name":"University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moeed","family":"Akbar","sequence":"additional","affiliation":[{"name":"University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mindy","family":"Huynh","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6434-5687","authenticated-orcid":false,"given":"Timothy","family":"Law","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smriti\u00a0K.","family":"Raychaudhuri","sequence":"additional","affiliation":[{"name":"University of California  Davis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siba P.","family":"Raychaudhuri","sequence":"additional","affiliation":[{"name":"University of California  Davis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian F.","family":"Volkman","sequence":"additional","affiliation":[{"name":"Medical College of Wisconsin  Milwaukee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9251-9907","authenticated-orcid":false,"given":"Neal L.","family":"Millar","sequence":"additional","affiliation":[{"name":"University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam T.","family":"Hwang","sequence":"additional","affiliation":[{"name":"University of California  Davis, Sacramento"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30952-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1314258"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60726-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380602"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60594-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)61134-5"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2016.1221923"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.13860"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2016.09.015"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/2040622318759282"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.14703"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.65"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3817"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-016-3500-x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.40150"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2817"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39732"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicines5030069"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.1.214"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2013.97"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071397"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6621"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1704958114"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425925"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003986"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1346-8138.14899"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.260"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101824"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101817"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1412679"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.6.2095.bloodjournal8762095"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1302\/2046-3758.612.BJR-2017-0112.R1"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2010.504716"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI37378"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar4317"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.13286"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.4161\/self.1.4.13904"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2017.12.006"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aam8834"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-1036-6"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-203643"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-019-0364-x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2019-215356"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2020.03.965"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOCI.0000040919.16739.44"}],"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.41882","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/art.41882","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/art.41882","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.41882","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T12:55:27Z","timestamp":1759928127000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.41882"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11]]},"references-count":46,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1002\/art.41882"],"URL":"https:\/\/doi.org\/10.1002\/art.41882","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11]]},"assertion":[{"value":"2020-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}