{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:07:08Z","timestamp":1788566828969,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"The National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["82122015"],"award-info":[{"award-number":["82122015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002799","name":"Capital Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002799","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Sj\u00f6gren's syndrome (SS) is a systemic autoimmune disease, and T cells play an important role in the initiation and perpetuation of the disease. In this study, we developed an immunotherapy for NOD\/LtJ mice with SS\u2010like symptoms by combining a transient depletion of CD4+ T cells with the administration of autoantigen\u2010specific peptide Ro480.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>NOD\/LtJ mice were treated with single anti\u2010CD4 monoclonal antibody (mAb) followed 2\u2009days later by a series of 6 intraperitoneal injections of Ro480\u2013494 every other day. Salivary flow rates were determined pre\u2010 and posttreatment once a week. Mice were euthanized 6\u2009weeks after the initial anti\u2010CD4 mAb treatment, salivary glands (SGs) were collected for analyses of histologic disease scores and inflammatory cell infiltration, polymerase chain reaction determination of genes was conducted, and flow cytometry analysis including major histocompatibility complex class II tetramer staining of immune cells was performed. In addition, adoptive transfer of Treg cells was administrated to investigate the function of the newly generating Treg cells in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The combination of anti\u2010CD4 mAb with autoantigen\u2010specific peptide Ro480 generated SSA\/Ro antigen\u2013specific Treg cells in vivo, which can suppress interferon\u2010\u03b3 production of CD4+ T cells and inflammation infiltration in SGs and maintain the function of SGs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings provide a new approach to generating antigen\u2010specific Treg cells in vivo for SS treatment, which may have implications for potential therapy for patients with SS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.42244","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:22:53Z","timestamp":1653351773000},"page":"1699-1705","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["In Vivo Generation of SSA\/Ro Antigen\u2013Specific Regulatory T Cells Improves Experimental Sj\u00f6gren's Syndrome in Mice"],"prefix":"10.1002","volume":"74","author":[{"given":"Junji","family":"Xu","sequence":"first","affiliation":[{"name":"Mucosal Immunology Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland, and Department of Periodontology School of Stomatology, Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section National Institute of Dental and Craniofacial Research, NIH  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Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Cain","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section National Institute of Dental and Craniofacial Research, NIH  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Bynum","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section National Institute of Dental and Craniofacial Research, NIH  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section National Institute of Dental and Craniofacial Research, NIH  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Han","sequence":"additional","affiliation":[{"name":"Mucosal Immunology Section National Institute of Dental and Craniofacial Research, NIH  Bethesda 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