{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:23:30Z","timestamp":1789604610184,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005564","name":"Gilead Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Anti\u2013citrullinated protein antibodies (ACPAs) are highly specific for rheumatoid arthritis (RA) and have long been regarded as pathogenic. Despite substantial in vitro evidence supporting this claim, reports investigating the proinflammatory effects of ACPAs in animal models of arthritis are rare and include mixed results. Here, we sequenced the plasmablast antibody repertoire of a patient with RA and functionally characterized the encoded ACPAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We expressed ACPAs from the antibody repertoire of a patient with RA and characterized their autoantigen specificities on antigen arrays and enzyme\u2010linked immunosorbent assays. Binding affinities were estimated by bio\u2010layer interferometry. Select ACPAs (n\u00a0=\u00a09) were tested in the collagen antibody\u2013induced arthritis (CAIA) mouse model to evaluate their effects on joint inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Recombinant ACPAs bound preferentially and with high affinity (nanomolar range) to citrullinated (cit) autoantigens (primarily histones and fibrinogen) and to auto\u2010cit peptidylarginine deiminase 4 (PAD4). ACPAs were grouped for in vivo testing based on their predominant cit\u2010antigen specificities. Unexpectedly, injections of recombinant ACPAs significantly reduced paw thickness and arthritis severity in CAIA mice as compared with isotype\u2010matched control antibodies (<jats:italic>P<\/jats:italic> \u2264\u20090.001). Bone erosion, synovitis, and cartilage damage were also significantly reduced (<jats:italic>P<\/jats:italic> \u2264\u20090.01). This amelioration of CAIA was observed for all the ACPAs tested and was independent of cit\u2010PAD4 and cit\u2010fibrinogen specificities. Furthermore, disease amelioration was more prominent when ACPAs were injected at earlier stages of CAIA than at later phases of the model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Recombinant patient\u2010derived ACPAs ameliorated CAIA. Their antiinflammatory effects were more preventive than therapeutic. This study highlights a potential protective role for ACPAs in arthritis.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/art42679-toc-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1002\/art.42679","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T09:19:00Z","timestamp":1692782340000},"page":"181-191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Anti\u2013Citrullinated Protein Antibodies With Multiple Specificities Ameliorate Collagen Antibody\u2013Induced Arthritis in a <scp>Time\u2010Dependent<\/scp> Manner"],"prefix":"10.1002","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4415-2615","authenticated-orcid":false,"given":"Alejandro M.","family":"Gomez","sequence":"first","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Camille","family":"Brewer","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Seung","family":"Moon","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman","family":"Acharya","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Kongpachith","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7357-9891","authenticated-orcid":false,"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaghayegh","family":"Jahanbani","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi H.","family":"Wong","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias V.","family":"Lanz","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zelda Z.","family":"Love","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, Stanford, and VA Palo Alto Health Care System  Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gundula","family":"Min\u2010Oo","sequence":"additional","affiliation":[{"name":"Gilead Sciences Inc.  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