{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:08:47Z","timestamp":1791497327002,"version":"4.3.4"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T00:00:00Z","timestamp":1758412800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005744","name":"Lastentautien Tutkimuss\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/501100005744","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004212","name":"P\u00e4ivikki ja Sakari Sohlbergin S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/501100004212","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Patients with juvenile idiopathic arthritis (JIA) frequently exhibit antinuclear antibodies (ANAs), but the specific antigen target recognized by them and the presence of additional autoantibody specificities in patients with JIA remains elusive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Plasma samples from 110 untreated patients with active JIA, as well as from 14 children with unspecified arthritis and 151 age\u2010 and sex\u2010matched healthy children, were analyzed with multiple modern clinical\u2010grade autoantibody assays, including automated indirect immunofluorescence to screen for ANAs with HEp\u20102 cells, and specific immune assays to detect reactivity to individual autoantigens. In addition, a HuProt proteome microarray was used to screen for novel autoantibody targets in plasma samples from five patients with ANA\u2010positive JIA and four ANA\u2010negative healthy controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Homogeneous nuclear ANA staining, indicating reactivity toward chromatin, was detected in most (61.8%) patients with JIA but rarely in healthy controls (2.6%;\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001). No antibody reactivity to specific nuclear antigens or other autoantigens associated with connective tissue diseases was detected. However, 20% of patients with JIA harbored antibodies against double\u2010stranded DNA (dsDNA)\u2013nucleosome complexes (compared with 2.6% of controls,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Finally, the proteome microarray revealed core histone H2A variant H2AFY, part of the nucleosome, to be the most widely recognized human protein by autoantibodies of patients with JIA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Autoantibody reactivity in JIA primarily targets chromatin, but the epitopes targeted are likely either posttranslationally modified or multimolecule epitopes, such as dsDNA\u2013nucleosome complexes, rather than epitopes on individual native proteins or purified dsDNA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.43315","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T03:25:47Z","timestamp":1752722747000},"page":"483-488","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Autoantibody Response Toward Chromatin in Patients With Juvenile Idiopathic Arthritis"],"prefix":"10.1002","volume":"78","author":[{"given":"Viola","family":"Pitk\u00e4nen","sequence":"first","affiliation":[{"name":"University of Eastern Finland  Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terhi","family":"Remes\u2010Pakarinen","sequence":"additional","affiliation":[{"name":"Kuopio University Hospital  Kuopio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"V\u00e4h\u00e4salo","sequence":"additional","affiliation":[{"name":"Oulu University Hospital and University of Oulu  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milja","family":"M\u00f6tt\u00f6nen","sequence":"additional","affiliation":[{"name":"University of Turku and Turku University Hospital  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minna\u2010Maija","family":"Gr\u00f6nlund","sequence":"additional","affiliation":[{"name":"University of Turku and Turku University Hospital  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miika","family":"Arvonen","sequence":"additional","affiliation":[{"name":"Kuopio University Hospital  Kuopio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Knip","sequence":"additional","affiliation":[{"name":"Tampere University Hospital, Tampere, and University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riitta","family":"Veijola","sequence":"additional","affiliation":[{"name":"Oulu University Hospital and University of Oulu  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorma","family":"Toppari","sequence":"additional","affiliation":[{"name":"University of Turku and Turku 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