{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T02:47:48Z","timestamp":1773715668114,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2025ZD0549800"],"award-info":[{"award-number":["2025ZD0549800"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Mutations in\n                      <jats:italic>ZBTB20<\/jats:italic>\n                      ,\u00a0 which encodes a transcription factor, are linked to epiphyseal dysplasia and articular degeneration in humans. This study investigates the role of ZBTB20 in regulating articular cartilage growth and integrity during postnatal development and its implications for early\u2010onset osteoarthritis (OA) in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We assessed the spatiotemporal expression of ZBTB20 in articular cartilage using immunostaining and generated an inducible cartilage\u2010specific\n                      <jats:italic>Zbtb20<\/jats:italic>\n                      knockout mouse model. The impacts of\n                      <jats:italic>Zbtb20<\/jats:italic>\n                      deletion on cartilage thickness, zonal organization, cellular proliferation, and apoptosis were analyzed. We employed histology, microcomputed tomography, in situ hybridization, RNA\u2010sequencing (RNA\u2010seq), and cleavage under targets and tagmentation (CUT&amp;Tag) to evaluate structural and molecular changes in knees from six to eight male mice per group.\n                      <jats:italic>ZBTB20<\/jats:italic>\n                      expression in human OA cartilage was analyzed using publicly available datasets.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ZBTB20 was expressed in postnatal developing and adult articular chondrocytes. Postnatal\n                      <jats:italic>Zbtb20<\/jats:italic>\n                      deletion resulted in progressive thickening of articular cartilage in knees, with a 1.9\u2013fold increase at two months of age, particularly in the deep and calcified zones. This was accompanied by chondrocyte overproliferation and differentiation defects, leading to early\u2010onset cartilage degeneration by six months. RNA\u2010seq and CUT&amp;Tag analyses revealed that ZBTB20 directly regulates a broad set of genes essential for cartilage growth, chondrocyte differentiation, and extracellular matrix organization. Moreover,\n                      <jats:italic>ZBTB20<\/jats:italic>\n                      expression was significantly reduced in aging\u2010related OA cartilage in both mice and humans, and inducible deletion of\n                      <jats:italic>Zbtb20<\/jats:italic>\n                      in adult cartilage resulted in severe spontaneous OA\u2010like changes in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>ZBTB20 is essential for postnatal articular cartilage development and homeostasis, with a protective role in aging\u2010related OA progression.<\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/art43393-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.43393","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T08:41:48Z","timestamp":1757925708000},"page":"630-643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>ZBTB20<\/scp>\n                    Regulating Postnatal Articular Cartilage Development and Homeostasis: Implications for Early\u2010Onset\u00a0Osteoarthritis"],"prefix":"10.1002","volume":"78","author":[{"given":"Xianhua","family":"Ma","sequence":"first","affiliation":[{"name":"Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Ministry of Education\u2010Shanghai Key Laboratory of Children's Environmental Health, Institute of Early Life Health, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenbang","family":"Qin","sequence":"additional","affiliation":[{"name":"Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology, Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunchun","family":"Wei","sequence":"additional","affiliation":[{"name":"Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Han","sequence":"additional","affiliation":[{"name":"Chu Hsien\u2010I Memorial Hospital &amp; 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