{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T08:04:07Z","timestamp":1775549047411,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["EQU202203014631"],"award-info":[{"award-number":["EQU202203014631"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003762","name":"Pasteur Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201022\u2010PEBI\u20100009 PEPR ACCREDIA"],"award-info":[{"award-number":["ANR\u201022\u2010PEBI\u20100009 PEPR ACCREDIA"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis & Rheumatology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"\n Objective<\/jats:title>\n The spontaneous K\/BxN mouse model of rheumatoid arthritis has been used extensively to study chronic inflammation, contribution of immune cells, and the primordial role of autoreactive antibodies in disease initiation and severity. Only the ubiquitous enzyme glucose\u20106\u2010phosphate isomerase (GPI) is the target of IgG autoantibodies secreted by autoreactive plasma cells and plasmablasts in K\/BxN mice. Strikingly, the appearance and evolution of these autoreactive IgG\u2010secreting cells (IgG\u2010SC) remain unstudied.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Here, we quantitatively and qualitatively investigated the plasmablast and plasma cell responses by measuring the affinity of their secreted antibody for GPI from single cells in cohorts of K\/BxN mice from 3 weeks to 87 weeks old.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Analysis of more than 36,000 individual IgG\u2010SC from spleen, popliteal lymph nodes, and bone marrow revealed high intercellular variability in affinity for GPI, with variations over three logs, with stable secretion rates over the life of the mice. Autoreactive IgG\u2010SC were detectable at 3 to 4 weeks and reached peak proportions of IgG\u2010SC at 35 weeks before stabilizing. High\u2010affinity anti\u2010GPI IgG\u2010SC appeared only transiently in the 6 to 9 weeks postnatal window, whereas low\u2010affinity IgG\u2010SC represented more than 80% of the response at all time points. Serum anti\u2010GPI IgG antibodies evolved in a similar kinetic manner, peaking in proportion to total IgG and in affinity for GPI at 35 weeks.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Our results report the dynamic nature of the autoimmune B cell response in the K\/BxN model, revealing a transient phase of antibody affinity maturation early in disease that allows high\u2010affinity antibody responses.<\/jats:p>\n \n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/art.43436","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T13:52:16Z","timestamp":1763128336000},"page":"821-829","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lifelong Evolution of Autoreactive Plasma Cell Numbers, Affinity, and Anatomic Location in Arthritic K\/\n BxN<\/scp>\n Mice"],"prefix":"10.1002","volume":"78","author":[{"given":"Thibault","family":"Vanhoucke","sequence":"first","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"},{"name":"Sorbonne Universit\u00e9, Coll\u00e8ge Doctoral Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Castrillon","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odile","family":"Richard\u2010Le Goff","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angga","family":"Perima","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matteo","family":"Broketa","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"},{"name":"Sorbonne Universit\u00e9, Coll\u00e8ge Doctoral Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew D.","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie, ESPCI Paris, PSL Research University, CNRS UMR8231 Chimie Biologie Innovation Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"England","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, CNRS UMR3528, Plateforme de Biophysique Mol\u00e9culaire Paris 75015 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois","family":"Huetz","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4709-8936","authenticated-orcid":false,"given":"Pierre","family":"Bruhns","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 de Paris, Unit of Antibodies in Therapy and Pathology, Inserm UMR1222 75015 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/90696"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000001006"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S41645"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.597959"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1522s81"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20011941"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003642"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912152106"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601776"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182549099"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060701"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001074107"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3964"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002942"}],"container-title":["Arthritis 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