{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:16:32Z","timestamp":1787904992225,"version":"build-2784847793"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000098","name":"Clinical Center","doi-asserted-by":"publisher","award":["1167415"],"award-info":[{"award-number":["1167415"]}],"id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["Z01 DE00695"],"award-info":[{"award-number":["Z01 DE00695"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015391","name":"Myositis Association","doi-asserted-by":"publisher","award":["1166727"],"award-info":[{"award-number":["1166727"]}],"id":[{"id":"10.13039\/100015391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp;amp; Rheumatology"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Myositis\u2010specific autoantibodies guide the diagnosis and classification of idiopathic inflammatory myopathies, but current immunoassays vary in accuracy, particularly for autoantibodies associated with interstitial lung disease. To compare the performance of the luciferase immunoprecipitation systems (LIPS) assay with line blot and immunoprecipitation (IP) and to evaluate whether antibody\u2010specific thresholds modify line blot concordance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed 279 sera with line blot results from the Johns Hopkins Myositis Center. LIPS was performed for anti\u2013Jo\u20101, anti\u2013PL\u20107, anti\u2013PL\u201012, anti\u2013MDA\u20105, anti\u2010EJ, and anti\u2010Ro52 autoantibodies. Line blot results were evaluated using standard thresholds (\u226536 and \u226571 arbitrary units [AU]). Assay performance was evaluated against IP (n = 141). In patients without IP (n = 138), clinical diagnoses were examined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LIPS showed excellent agreement with IP (\u03ba = 0.90, area under the receiver operating characteristic curve 0.935), outperforming line blot. Line blot cutoffs influenced performance: \u226536 AU increased sensitivity (92.9%) but reduced specificity (73.8%), whereas \u226571 AU improved specificity (92.9%) but lowered sensitivity (82.8%). Antibody\u2010specific thresholds modified concordance but remained quantitatively inferior to LIPS. In patients without IP, concordance between LIPS and line blot was 89% at \u226536 AU and 83% at \u226571 AU. LIPS\u2010positive cases were enriched for antisynthetase syndrome and dermatomyositis. Among discordant cases (line blot positive and LIPS negative), most were clinically classified as inclusion body myositis (5 of 11). For anti\u2010Ro52, discrepancies were most frequent at titers &lt;36 AU.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LIPS provides quantitative discrimination, strong concordance with IP, and improved performance compared with line blot, particularly at low to intermediate titers. By reducing misclassification and complementing antibody\u2010specific thresholds, LIPS has the potential to enhance clinical stratification in patients with myositis.<\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/art70103-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.70103","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:18:36Z","timestamp":1773001116000},"page":"1952-1960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Improved Detection of Myositis\u2010Specific Autoantibodies Using Luciferase Immunoprecipitation Systems Assay: Comparison With Line Blot and Conventional Immunoprecipitation"],"prefix":"10.1002","volume":"78","author":[{"given":"Peter D.","family":"Burbelo","sequence":"first","affiliation":[{"name":"Adeno\u2010Associated Virus Biology Section, National Institute of Dental and Craniofacial Research NIH  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6338-9218","authenticated-orcid":false,"given":"Iago","family":"Pinal\u2010Fernandez","sequence":"additional","affiliation":[{"name":"Muscle Disease Section, National 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