{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:10:28Z","timestamp":1790302228691,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC 2167 (CD\u20105)"],"award-info":[{"award-number":["EXC 2167 (CD\u20105)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 2633 (A6)"],"award-info":[{"award-number":["GRK 2633 (A6)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis & Rheumatology"],"abstract":"\n Objective<\/jats:title>\n Regeneration and expansion of Treg by low\u2010dose interleukin\u20102 (IL\u20102) therapy is considered a potential treatment strategy for a wide range of autoimmune diseases. To provide a pathophysiologically\u2010based rationale for low\u2010dose IL\u20102 therapy, we investigated whether reversible defects in the Treg\u2013IL\u20102 axis emerge in inflammatory myopathies.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n CD4+ T cell subsets from patients with polymyositis (PM) or dermatomyositis (DM) (n\u00a0=\u00a020) and healthy controls (HC) (n\u00a0=\u00a019) and peripheral blood mononuclear cells (PBMC) from eight patients that were stimulated in vitro for 24 hours with different concentrations of recombinant human IL\u20102 were analyzed by multicolor flow cytometry. Messenger RNA (mRNA) expressions of\n IL2RA<\/jats:italic>\n ,\n IL2RB<\/jats:italic>\n ,\n IL2RB<\/jats:italic>\n ,\n ENTPD1<\/jats:italic>\n ,\n IKZF2<\/jats:italic>\n , and\n CTLA4<\/jats:italic>\n were quantified by real\u2010time polymerase chain reaction in IL\u20102 stimulated PBMC (n\u00a0=\u00a06). Two patients with refractory PM\/DM were treated with low\u2010dose IL\u20102 therapy for eight weeks and monitored for clinical responses and changes in Treg subsets.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Frequencies of Treg expressing CD25 at high levels (CD25hi Treg) and of CXCR5+ Treg were reduced in patients with PM\/DM compared with HC (\n P<\/jats:italic>\n \u00a0=\u00a00.0052;\n P<\/jats:italic>\n \u00a0=\u00a00.0661), particularly in active myositis (\n P<\/jats:italic>\n \u00a0=\u00a00.0036;\n P<\/jats:italic>\n \u00a0=\u00a00.0335). Stimulation with low doses of IL\u20102 selectively enhanced expression of CD25 molecules by Treg, leading to an increase in the CD25hi Treg subset (\n P<\/jats:italic>\n \u2009<\u20090.05) and augmented mRNA expression of several immunoregulatory molecules (\n P<\/jats:italic>\n \u2009<\u20090.05). Low\u2010dose IL\u20102 therapy induced decreases in muscle enzymes that were accompanied by a sustained expansion of CD25+ Treg.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Our data suggest that shortage of IL\u20102 is pathophysiologically relevant in PM\/DM. Recovery and expansion of Treg by low\u2010dose IL\u20102 therapy could thus be a promising targeted treatment option.<\/jats:p>\n \n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/art.70151","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:44:58Z","timestamp":1774503898000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interleukin\u20102 Deprived State of Regulatory T cells and Their Recovery by Low\u2010Dose Interleukin\u20102 in Patients With Inflammatory Myopathies"],"prefix":"10.1002","author":[{"given":"Justus","family":"Ohmes","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology University Medical Center Schleswig\u2010Holstein \/ University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa R.","family":"Monne","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology University Medical Center Schleswig\u2010Holstein \/ University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Comd\u00fchr","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology University Medical Center Schleswig\u2010Holstein \/ University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fynn","family":"Gerlach","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology University Medical Center Schleswig\u2010Holstein \/ University of L\u00fcbeck L\u00fcbeck 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