{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T11:47:57Z","timestamp":1776858477042,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100015993","name":"Kowa Company","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015993","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K17430"],"award-info":[{"award-number":["JP20K17430"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20H03720"],"award-info":[{"award-number":["JP20H03720"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP22K08528"],"award-info":[{"award-number":["JP22K08528"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp; Rheumatology"],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Immune complexes (ICs), formed by autoantigen and autoantibody, play a pathogenic role in systemic autoimmune diseases through stimulation of Fc\u03b3 receptors (Fc\u03b3R). However, studies investigating bioactivity of circulating ICs across various diseases remain limited. We aimed to quantify bioactivity of serum ICs using an Fc\u03b3R\u2010reporter cell assay and examine their association with disease activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We assessed the bioactivity of serum ICs using the Fc\u03b3RIIIA\u2010reporter assay. Serum samples were obtained from healthy controls (n = 56), infectious disease controls (n = 12), systemic lupus erythematosus (SLE) (n = 175), Sj\u00f6gren disease (n = 71), mixed connective tissue disease (n = 22), idiopathic inflammatory myopathies (n = 73), systemic sclerosis (n = 107), and rheumatoid arthritis (n = 51).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The bioactivity of ICs was significantly elevated across various systemic autoimmune diseases and associated with disease activity. In SLE, bioactivity of ICs was notably elevated compared with other diseases. The bioactivity in patients with SLE reflected disease activity with sensitivity of 81% and specificity of 69%. In patients with low anti\u2013double\u2010stranded DNA antibody levels, those with high IC bioactivity were at higher risk of clinical symptoms including nephritis, arthritis, and skin involvement, providing additive value in assessing SLE activity. Longitudinal analysis also showed that the Fc\u03b3RIIIA\u2010reporter assay more sensitively reflected flares than conventional serological markers, and those who achieved a low Fc\u03b3RIIIA\u2010reporter response within the initial 3 months tended to achieve remission at 24 months (90% vs 75%,\n                      <jats:italic>P<\/jats:italic>\n                      = 0.56).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The Fc\u03b3RIIIA\u2010reporter assay enables precise quantification of pathogenic bioactivity of ICs in various systemic autoimmune diseases, providing complementary information to existing serological markers.<\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/art70159-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.70159","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T10:12:46Z","timestamp":1775211166000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Immune Complexes Shape the Pathogenicity of Autoantibodies in Systemic Autoimmune Diseases: Insights From an\n                    <scp>Fc\u03b3RIIIA<\/scp>\n                    \u2010Reporter Assay"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1358-7069","authenticated-orcid":false,"given":"Koji","family":"Suzuki","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Keio University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3281-6595","authenticated-orcid":false,"given":"Masaru","family":"Takeshita","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Keio University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Imai","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine Keio University School of Medicine  Tokyo 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