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We then analyzed BD and healthy control CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN using RNA sequencing (n\u00a0=\u00a07) and measured neutrophil extracellular trap (NET) production, which were used to stimulate vascular endothelial cells (VECs) for RNA\u2010sequencing analysis. Differentially expressed proteins in NETs were determined by mass spectrometry. Senescence, senescence\u2010associated secretory phenotype (SASP), and PMN chemotaxis of VECs treated with BD CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN\u2010derived NETs were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Transcriptome analysis revealed BD PMN overexpressed CD177. CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN subset was expanded in active and severe systemic BD and accumulated in BD aortic walls. Enhanced NETosis and oxidative phosphorylation in BD CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN were noted in RNA sequencing, which were confirmed in both resting and activated BD CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN. BD CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN\u2010derived NETs promoted senescence, SASP, and PMN chemotaxis in VECs using RNA\u2010sequencing analysis, which were validated by up\u2010regulated IL\u20106, CXCL8, ICAM\u20101, p21, SA\u2010\u03b2\u2010gal, and cell proliferation arrest. Mass spectrometry identified that BD CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN\u2010derived NETs contained more histone 3.1 (H3.1). H3.1 induced senescence, SASP, and PMN chemotaxis in VECs via Toll\u2010like receptor 4 signaling, which was attenuated by senolytics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Increased CD177\n                      <jats:sup>+<\/jats:sup>\n                      PMN in patients with BD promotes senescence and SASP in VECs through secreting H3.1\u2010enriched NETs, which recruit PMN and collectively contribute to vasculitis in BD.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.70217","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T10:27:03Z","timestamp":1778495223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CD177<\/scp>\n                    <sup>+<\/sup>\n                    Neutrophils Promote Endothelial Senescence Through Extracellular Traps in Beh\u00e7et Disease"],"prefix":"10.1002","author":[{"given":"Xiaoou","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"National Clinical Research Center for Dermatologic and Immunologic Diseases Ministry of Science &amp; Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"National Clinical Research Center for Dermatologic and Immunologic Diseases Ministry of Science &amp; Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luxi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"National Clinical Research Center for Dermatologic and Immunologic Diseases Ministry of Science &amp; Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"National Clinical Research Center for Dermatologic and Immunologic Diseases Ministry of Science &amp; Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Clinical Immunology Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing China"},{"name":"National Clinical Research Center for Dermatologic and Immunologic Diseases Ministry of Science &amp; Technology  Beijing 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