{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T01:02:45Z","timestamp":1784595765005,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T00:00:00Z","timestamp":1784505600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012295","name":"NIHR Newcastle Biomedical Research Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012295","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp;amp; Rheumatology"],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>CD4 T cells specific for citrullinated (cit) peptides are key players in rheumatoid arthritis (RA) immunopathogenesis. Characterizing these cells and identifying features of healthy and RA\u2010associated autoreactivity will provide valuable insight into disease mechanisms and form the basis of immune state biomarkers to facilitate the next generation of RA treatments. We sought to characterize these cells in a UK early arthritis cohort.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cit\u2010peptide\u2013specific (autoreactive) CD4 T cells from human leukocyte antigen (HLA)\u2010DRB1 04:01\u2013positive healthy controls (HCs; n\u00a0=\u00a09), patients with RA at different disease stages (n\u00a0=\u00a017 pretreatment and 13 receiving treatment), and six at\u2010risk individuals were assessed using major histocompatibility complex II peptide tetramers combined with spectral flow cytometry. Autoreactive T cell frequency and phenotype were studied, with a particular focus on the memory CD4 T cell compartment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>There was no statistically significant difference in autoreactive T cell frequency between comparator groups. In HCs, however, autoreactive T cells were more likely to be naive. In early pretreatment RA, autoreactive memory T cells demonstrated down\u2010regulation of CD27 and CD28 and expression of the fractalkine receptor CX3CR1, features that have been linked to CD4 cytotoxicity. The proportion of CD27\u2010negative autoreactive cells decreased following treatment, and RA\u2010associated features were evident in some at\u2010risk donors before RA onset.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In RA, CD4 T cell autoreactivity is characterized by a surface phenotype that suggests in vivo activation and the potential to acquire cytotoxic capacity, whereas healthy autoreactivity demonstrates naivety. These observations suggest maturing autoreactivity in RA and may provide novel tools for monitoring disease progression and tolerance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.70235","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T12:16:57Z","timestamp":1780316217000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Autoreactivity Versus Autoimmunity: Characteristics of Autoantigen\u2010Specific\n                    <scp>CD4<\/scp>\n                    T Cells in Rheumatoid Arthritis and Health"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2532-2965","authenticated-orcid":false,"given":"James A.","family":"Stanway","sequence":"first","affiliation":[{"name":"Translational and Clinical Research Institute, Faculty of Medical Sciences Newcastle University  Newcastle upon Tyne United Kingdom"},{"name":"Department of Rheumatology The 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