{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T02:38:10Z","timestamp":1792550290529,"version":"4.5.0"},"reference-count":0,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["acrjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthritis &amp;amp; Rheumatology"],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Psoriatic arthritis (PsA) is a chronic immune\u2010mediated arthritis affecting approximately 30% of individuals with psoriasis. The mechanisms driving PsA joint inflammation remain incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To address this gap, we performed multimodal single\u2010cell sequencing of immune cells from matched peripheral blood and synovial fluid (SF) from 6 PsA patients, with comparisons to a rheumatoid arthritis (RA) patient and 6 healthy controls (HC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      SF CD4 and CD8 T cells displayed elevated expression of\n                      <jats:italic>TOX<\/jats:italic>\n                      ,\n                      <jats:italic>PDCD1<\/jats:italic>\n                      ,\n                      <jats:italic>CTLA4<\/jats:italic>\n                      ,\n                      <jats:italic>GZMK<\/jats:italic>\n                      ,\n                      <jats:italic>CXCR3<\/jats:italic>\n                      , and\n                      <jats:italic>CXCR6<\/jats:italic>\n                      , reflecting a tissue\u2010specific activation state. CD4 T cell sub\u2010clustering revealed clonally expanded populations enriched for\n                      <jats:italic>GZMK<\/jats:italic>\n                      and cytotoxic gene programs, suggesting antigen\u2010driven activation. In contrast, CD8 T cell clonality showed a significant correlation with\n                      <jats:italic>GZMK<\/jats:italic>\n                      expression, however\n                      <jats:italic>GZMK<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      CD8 T cells in the joint exhibited a transcriptional signature consistent with functional exhaustion. Concurrently, SF was enriched for CD141\n                      <jats:sup>+<\/jats:sup>\n                      , CD1c\n                      <jats:sup>+<\/jats:sup>\n                      , CCR7\n                      <jats:sup>+<\/jats:sup>\n                      , and SIGLEC6\n                      <jats:sup>+<\/jats:sup>\n                      dendritic cells (DCs), characterized by increased\n                      <jats:italic>CXCL16<\/jats:italic>\n                      expression and antigen presentation pathways. Flow cytometry and ELISA in an additional 25 PsA patients and 16 HCs confirmed protein\u2010level enrichment of key chemokines and GZMK\n                      <jats:sup>+<\/jats:sup>\n                      T cells. Cell\u2013cell communication analysis highlighted\n                      <jats:italic>CXCR6\u2013CXCL16<\/jats:italic>\n                      and\n                      <jats:italic>CXCR3\u2013CXCL10<\/jats:italic>\n                      chemokine axes as mediators of immune cell recruitment and retention, while\n                      <jats:italic>in vitro<\/jats:italic>\n                      assays showed enhanced migration of PsA SF T cells toward a CXCL16 gradient relative to paired blood.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Together, these findings suggest that clonally expanded\n                      <jats:italic>GZMK<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      CD4 T cells in PsA are cytotoxic and antigen\u2010responsive, contributing to joint inflammation following recruitment by\n                      <jats:italic>CXCL16<\/jats:italic>\n                      \u2010producing dendritic cells.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/art70371-toc-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.70371","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:46:46Z","timestamp":1792547206000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010cell analysis reveals CXCL16\n                    <sup>+<\/sup>\n                    dendritic cells and clonally expanded GZMK\n                    <sup>+<\/sup>\n                    CD4 T cells driving joint inflammation in psoriatic arthritis"],"prefix":"10.1002","author":[{"given":"Briana J.","family":"Mullins","sequence":"first","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Shwetar","sequence":"additional","affiliation":[{"name":"Division of Precision Medicine, Department of Medicine NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Herrera","sequence":"additional","affiliation":[{"name":"Immunology and Microbial Pathogenesis Program Weill Cornell Graduate School of Medical Sciences  New York NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Youzhi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences University of Connecticut Health Center  Farmington CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharine Lu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Jim\u00e9nez","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Tumang","sequence":"additional","affiliation":[{"name":"Division of Precision Medicine, Department of Medicine NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni P.","family":"Mimitou","sequence":"additional","affiliation":[{"name":"Immunai  New York NY 10016 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Sahimi","sequence":"additional","affiliation":[{"name":"Division of Precision Medicine, Department of Medicine NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhina","family":"Medina","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7119-8136","authenticated-orcid":false,"given":"Rebecca H.","family":"Haberman","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parvathy V.","family":"Girija","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"},{"name":"Rocket Pharmaceuticals, Inc.,\u00a0R&amp;D and Corporate Headquarters  Cranbury NJ 08512"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4289-7931","authenticated-orcid":false,"given":"Thomas","family":"Boehringer","sequence":"additional","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7638-6382","authenticated-orcid":false,"given":"Timothy C.","family":"Borbet","sequence":"additional","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Eichman","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsty","family":"Hillier","sequence":"additional","affiliation":[{"name":"Department of Pediatrics NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Bajwa","sequence":"additional","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celiane Cardoso","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan R.","family":"Schwab","sequence":"additional","affiliation":[{"name":"Department of Cell Biology New York University Grossman School of Medicine  New York NY 10016 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7080-8118","authenticated-orcid":false,"given":"Mukundan G.","family":"Attur","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Ruggles","sequence":"additional","affiliation":[{"name":"Division of Precision Medicine, Department of Medicine NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1072-6994","authenticated-orcid":false,"given":"Jos\u00e9 U.","family":"Scher","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine and Psoriatic Arthritis Center NYU Grossman School of Medicine  New York NY USA"},{"name":"NYU Colton Center for Autoimmunity, Department of Medicine, NYU Langone Health  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4843-3791","authenticated-orcid":false,"given":"Sergei B.","family":"Koralov","sequence":"additional","affiliation":[{"name":"Department of Pathology NYU Grossman School of Medicine  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,20]]},"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.70371","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.70371","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:46:47Z","timestamp":1792547207000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.70371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,20]]},"references-count":0,"alternative-id":["10.1002\/art.70371"],"URL":"https:\/\/doi.org\/10.1002\/art.70371","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,20]]},"assertion":[{"value":"2025-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"art.70371"}}