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Moreover, COVID\u201019 positive cadavers have been rejected by medical schools, adversely impacting the continuum of medical education. Herein, the viral genome abundance in tissues from four COVID\u201019 positive donors before and after embalming were compared. Tissue samples were collected from the lungs, liver, spleen, and brain both pre\u2010 and postembalming. The possible presence of infectious COVID\u201019 was determined by inoculating human tissue homogenates onto a monolayer of human A549\u2010hACE2 cells and observing for cytopathic effects up to 72\u2009h postinoculation. A real\u2010 time quantitative reverse transcription polymerase chain reaction was performed to quantify COVID\u201019 present in culture supernatants. Fully intact viral genome sequence was possible to obtain in samples with higher levels of virus, even several days postmortem. The embalming procedure described above substantially reduces the abundance of viable COVID\u201019 genomes in all tissues, sometimes even to undetectable levels. However, in some cases, COVID\u201019 RNA can still be detected, and a cytopathic effect can be seen both pre\u2010 and postembalmed tissues. This study suggests that embalmed COVID\u201019 positive cadavers might be used safely with appropriate precautions followed in gross anatomy laboratories and in clinical and scientific research. Deep lung tissue is the best specimen to test for the virus. If the tests on the lung tissues are negative, there is a very low likelihood that other tissues will show positive results.<\/jats:p>","DOI":"10.1002\/ase.2300","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:15:21Z","timestamp":1687133721000},"page":"802-813","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quantification and persistence of COVID\u201019 virus in recently deceased individuals before and after embalming"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1435-1819","authenticated-orcid":false,"given":"Lorenzo","family":"Gitto","sequence":"first","affiliation":[{"name":"Department of Pathology Cook County Medical Examiner's Office  Chicago Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank A.","family":"Middleton","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and 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