{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:51:11Z","timestamp":1778568671064,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,4,29]],"date-time":"2010-04-29T00:00:00Z","timestamp":1272499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry \u0097 An Asian Journal"],"published-print":{"date-parts":[[2010,5,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The core\u2010shell boronic\u2010acid functionalized nanoparticles SnO<jats:sub>2<\/jats:sub>@Poly(HEMA\u2010<jats:italic>co<\/jats:italic>\u2010St\u2010<jats:italic>co<\/jats:italic>\u2010VPBA) are designed for selectively enriching glycopeptides, followed by matrix\u2010assisted laser desorption\/ionization mass spectrometry (MALDI\u2010MS) analysis. Such 60\u2005nm sized core\u2010shell nanoparticles are prepared by means of copolymerization between 2\u2010hydroxyethyl methacrylate (HEMA) grafted on SnO<jats:sub>2<\/jats:sub> nanoparticles, styrene, and 4\u2010vinylphenylboronic acid (VPBA). All of the synthesis procedures are completed within 3\u2005h. Cyclic boronate esters form between boronic\u2010acid groups on the polymer chains and <jats:italic>cis<\/jats:italic>\u2010diol groups on glycopeptides, and thus almost all intact glycopeptides from low\u2010abundant horseradish peroxidase (HRP) and bovine asialofetuin (ASF) are enriched with high selectivity and efficiency. After enrichment, both intact N\u2010 and O\u2010glycopeptides are characterized by multistage MS. Furthermore, we successfully apply this method to the human serum sample for characterizing the target glycoproteins haptoglobin and alpha\u20101\u2010acid\u2010glycoprotein. The present selective enriching method followed by multistage\u2010MS analysis is proven to be a good choice for routine glycopeptide characterization.<\/jats:p>","DOI":"10.1002\/asia.200900542","type":"journal-article","created":{"date-parts":[[2010,3,16]],"date-time":"2010-03-16T15:20:55Z","timestamp":1268752855000},"page":"1185-1191","source":"Crossref","is-referenced-by-count":24,"title":["SnO<sub>2<\/sub>@Poly(HEMA\u2010<i>co<\/i>\u2010St\u2010<i>co<\/i>\u2010VPBA) Core\u2010shell Nanoparticles Designed for Selectively Enriching Glycopeptides Followed by MALDI\u2010MS Analysis"],"prefix":"10.1002","volume":"5","author":[{"given":"Wen\u2010Wen","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Ning","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Feng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan\u2010Ming","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao\u2010Jie","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng\u2010Yuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,29]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.08.019"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001534200"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1460"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1739"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058890"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2006.10.005"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050275j"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac049062o"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj019"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt829"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac048751x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt827"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T600046-MCP200"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200702919"},{"key":"e_1_2_6_14_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200702919"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi801640n"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac801704j"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(87)90026-X"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.06.028"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1039\/b808800d"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac801912t"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200705942"},{"key":"e_1_2_6_21_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200705942"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm060432u"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.43.5109"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4020(98)00469-4"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr070401e"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac001404c"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1039\/b713816d"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1546"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960610)10:8<932::AID-RCM595>3.0.CO;2-X"},{"key":"e_1_2_6_30_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0021-9258(19)57364-1","volume":"263","author":"Yet M. 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