{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T01:11:44Z","timestamp":1776474704054,"version":"3.51.2"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T00:00:00Z","timestamp":1345507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chemistry \u0097 An Asian Journal"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sugar chains are important molecules in cellular recognition and signaling, and quantum dots (QDs) are a very powerful tool for in vitro and in vivo imaging. Herein, we report the preparation of stable sugar\u2010chain\u2010immobilized fluorescent nanoparticles (SFNPs) and their application to the analysis of sugar\u2010chain\u2013protein interactions and cellular imaging. SFNPs were easily prepared by mixing CdTe\/CdS core\/shell QDs with our previously developed sugar\u2010chain\u2013ligand conjugates. The obtained SFNPs were very stable and could be stored for several months. In the binding analysis, \u03b2\u2010galactose\u2010 and \u03b1\u2010glucose\u2010immobilized SFNPs were specifically interacted with <jats:italic>Ricinus communis<\/jats:italic> agglutinin\u2005I and concanavalin\u2005A, respectively, and made into aggregates. The binding interaction was detected visually, fluorescently, or both. In the experiment for cellular imaging, it was found that SFNPs were predominantly taken up by human hepatocyto carcinoma cells (HepG2), suggesting the possible usage of our designed SFNPs for various biochemical analyses of sugar chains.<\/jats:p>","DOI":"10.1002\/asia.201200362","type":"journal-article","created":{"date-parts":[[2012,8,21]],"date-time":"2012-08-21T04:18:25Z","timestamp":1345522705000},"page":"2678-2682","source":"Crossref","is-referenced-by-count":19,"title":["Stable Sugar\u2010Chain\u2010Immobilized Fluorescent Nanoparticles for Probing Lectin and Cells"],"prefix":"10.1002","volume":"7","author":[{"given":"Hiroyuki","family":"Shinchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Wakao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sho","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiko","family":"Mochizuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Kuwabata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Suda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,8,21]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/3.2.97"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.271.5251.933"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.152463399"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3757(20011119)113:22<4254::AID-ANGE4254>3.0.CO;2-D"},{"key":"e_1_2_6_4_3","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3773(20011119)40:22<4128::AID-ANIE4128>3.0.CO;2-S"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104274"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200500786"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.07.014"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5385.2016"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm0107878"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5385.2013"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja048792a"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200400137"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/la800066g"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2004.12.002"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200600496"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500112"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc060204q"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja807711w"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc0600620"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2005.11.016"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvn038"},{"key":"e_1_2_6_22_2","first-page":"671","volume":"53","author":"Zhang X.","year":"2012","journal-title":"Polym. 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