{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:41:08Z","timestamp":1762252868972,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,12,4]],"date-time":"2018-12-04T00:00:00Z","timestamp":1543881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010890","name":"Chinese Government Scholarship","doi-asserted-by":"publisher","award":["2017_AU0001"],"award-info":[{"award-number":["2017_AU0001"]}],"id":[{"id":"10.13039\/501100010890","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001785","name":"Flinders University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001785","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["aces.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry \u0097 An Asian Journal"],"published-print":{"date-parts":[[2019,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper employs a specially designed aggregation\u2010induced emission fluorogen (AIEgen) to in vivo visualize the process of Hg<jats:sup>2+<\/jats:sup> bioaccumulation in a common species of freshwater zooplankton <jats:italic>Daphnia carinata<\/jats:italic> (<jats:italic>D.\u2005carinata<\/jats:italic>) by two methods, direct Hg<jats:sup>2+<\/jats:sup> absorption and ingestion of Hg<jats:sup>2+<\/jats:sup> contaminated food. We analysed the relevance between photoluminescence (PL, I<jats:sub>595<\/jats:sub>\/I<jats:sub>480<\/jats:sub>) ratios and Hg<jats:sup>2+<\/jats:sup> (C<jats:sub>Hg2+<\/jats:sub>) and developed the master curve for Hg<jats:sup>2+<\/jats:sup> determination based on measuring the PL intensity of the solution. Meanwhile, fluorescent image analysis showed that the major recipient organs of Hg<jats:sup>2+<\/jats:sup> in <jats:italic>D. carinata<\/jats:italic> were the compound eyes and carapace, followed by the intestine and shell gland, but not the brain or heart. The response of <jats:italic>D.\u2005carinata<\/jats:italic> to Hg<jats:sup>2+<\/jats:sup> via uptake from surrounding water differed distinctly from that through food intake of the algae (<jats:italic>Euglena gracilis<\/jats:italic>) contaminated by mercury. When Hg<jats:sup>2+<\/jats:sup> was encapsulated by algae membrane, no fluorescence was detected, and the carapace morphology remained intact after ingesting algae for 80\u2005min, in contrast to the rapid carapace deformation by direct Hg<jats:sup>2+<\/jats:sup> absorption. The <jats:italic>D.\u2005carinata<\/jats:italic> showed higher mortality by direct Hg<jats:sup>2+<\/jats:sup> immersion than via food ingestion. The reason for the high mortality after Hg<jats:sup>2+<\/jats:sup> immersion was possibly due to carapace deformity after chemical reaction between chitin and mercury, but the biochemical pathway leading to morality needs further study.<\/jats:p>","DOI":"10.1002\/asia.201801538","type":"journal-article","created":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T04:49:05Z","timestamp":1542084545000},"page":"796-801","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["In vivo Visualization of the Process of Hg<sup>2+<\/sup> Bioaccumulation in Water Flea <i>Daphnia carinata<\/i> by a Novel Aggregation\u2010Induced Emission Fluorogen"],"prefix":"10.1002","volume":"14","author":[{"given":"Tao","family":"He","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Southwest University  Chongqing 400715 China"},{"name":"College of Science and Engineering Flinders University  South Australia 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