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Abnormal H<jats:sub>2<\/jats:sub>S levels have been associated with a range of conditions, including Parkinson's and Alzheimer's diseases, cardiovascular and renal diseases, bacterial and viral infections, as well as cancer. Therefore, fast and sensitive H<jats:sub>2<\/jats:sub>S detection is of significant clinical importance. Fluorescent H<jats:sub>2<\/jats:sub>S probes hold great potential among the currently developed detection methods because of their high sensitivity, selectivity, and biocompatibility. However, many proposed probes do not provide a gold standard for proper use and selection. Consequently, issues arise when applying the probes in different conditions. Therefore, we systematically evaluated four commercially available probes (WSP\u20101, WSP\u20105, CAY, and P3), considering their detection range, sensitivity, selectivity, and performance in different environments. 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