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Fluorescent turn\u2010on H<jats:sub>2<\/jats:sub>S probes have been developed for use <jats:italic>in cellulo<\/jats:italic> and <jats:italic>in\u2005vivo<\/jats:italic>, but the membrane permeability of these probes can lead to probe leakage and signal attenuation over time. Here we report a cell trappable fluorescent probe for H<jats:sub>2<\/jats:sub>S, <jats:bold>CT\u2010MeRhoAz<\/jats:bold>, which is based on a methylrhodolazide scaffold derivatized with an acetoxymethyl ester group. Prior to ester cleavage, the <jats:bold>CT\u2010MeRhoAz<\/jats:bold> probe generates a 2500\u2010fold turn\u2010on response to H<jats:sub>2<\/jats:sub>S, which is enhanced to a 3000\u2010fold response for the carboxylic acid form of the probe. Additionally, the probe is highly selective for H<jats:sub>2<\/jats:sub>S over other biologically relevant sulfur, oxygen, and nitrogen\u2010based analytes. Live cell imaging experiments confirmed the biocompatibility of <jats:bold>CT\u2010MeRhoAz<\/jats:bold> and also that it is cell trappable, unlike the parent <jats:bold>MeRhoAz<\/jats:bold> scaffold.<\/jats:p>","DOI":"10.1002\/asia.202200426","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T15:13:21Z","timestamp":1655133201000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A Cell Trappable Methyl Rhodol\u2010Based Fluorescent Probe for Hydrogen Sulfide Detection"],"prefix":"10.1002","volume":"17","author":[{"given":"Kaylin G.","family":"Fosnacht","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Materials Science Institute Knight Campus for Accelerating Scientific Impact Institute of Molecular Biology University of Oregon  Eugene OR 97403 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew D.","family":"Hammers","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Materials Science Institute Knight Campus for Accelerating Scientific Impact Institute of Molecular Biology University of Oregon  Eugene OR 97403 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary S.","family":"Earp","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Materials Science Institute Knight Campus for Accelerating Scientific Impact Institute of Molecular Biology University of Oregon  Eugene OR 97403 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annie K.","family":"Gilbert","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Materials Science Institute Knight Campus for Accelerating Scientific Impact Institute of Molecular Biology University of Oregon  Eugene OR 97403 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3604-653X","authenticated-orcid":false,"given":"Michael D.","family":"Pluth","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Materials Science Institute Knight Campus for Accelerating Scientific Impact Institute of Molecular Biology University of Oregon  Eugene OR 97403 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"e_1_2_8_1_1","unstructured":"\u00a0"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7096"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.11.019"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.011304"},{"key":"e_1_2_8_5_1","unstructured":"\u00a0"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00576.2009"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvp111"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2011"},{"key":"e_1_2_8_9_1","unstructured":"\u00a0"},{"key":"e_1_2_8_10_2","first-page":"459","volume":"121","author":"Whiteman M.","year":"2011","journal-title":"Chem. 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