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Differentiating ZIKV from other arboviruses such as dengue virus (DENV) is crucial for effective diagnosis and treatment. This study presents the development of a biosensor for detecting the ZIKV non\u2010structural protein 1 (NS1) using gold nanoparticles (AuNPs) functionalized with monoclonal antibodies employing dynamic light scattering (DLS). The biosensor named <jats:bold>ZINS1\u2010mAb\u2010AuNP<\/jats:bold> exhibited specific binding to the ZIKV NS1 protein, demonstrating high colloidal stability indicated by a hydrodynamic diameter (D<jats:sub>H<\/jats:sub>) of 140\u2005nm, detectable via DLS. In the absence of the protein, the high ionic strength medium caused particle aggregation. This detection method showed good sensitivity and specificity, with a limit of detection (LOD) of 0.96\u2005\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>, and avoided cross\u2010reactivity with DENV2 NS1 and SARS\u2010CoV\u20102 spike proteins. 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