{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:31:29Z","timestamp":1786977089459,"version":"build-2736575974"},"reference-count":79,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T00:00:00Z","timestamp":1737676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["aces.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry \u0097 An Asian Journal"],"published-print":{"date-parts":[[2025,5,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, we have designed and developed a cationic bolaform C\n                    <jats:sub>12<\/jats:sub>\n                    \u2010(2,3\u2010dihydroxy\u2010N, N\u2010dimethyl\u2010N\u2010(2\u2010ureidoethyl)propan\u20101\u2010aminium chloride)\n                    <jats:sub>2<\/jats:sub>\n                    (C\n                    <jats:sub>12<\/jats:sub>\n                    (DDUPAC)\n                    <jats:sub>2<\/jats:sub>\n                    ) that is derived from biocompatible molecules. The bolaform C\n                    <jats:sub>12<\/jats:sub>\n                    (DDUPAC)\n                    <jats:sub>2<\/jats:sub>\n                    has hydroxyl (OH) functionality at both the cationic head groups. The impact of head group structure on the self\u2010assembly and effectiveness of gene transfection and antimicrobial activity was investigated and compared with that of the hydrochloride salt C\n                    <jats:sub>12<\/jats:sub>\n                    \u2010(N, N\u2010dimethyl\u2010N\u2010(2\u2010ureidoethan\u20101\u2010aminium chloride)\n                    <jats:sub>2<\/jats:sub>\n                    (C\n                    <jats:sub>12<\/jats:sub>\n                    (DUAC)\n                    <jats:sub>2<\/jats:sub>\n                    ) of its precursor molecule. The formation of spherical as well as rod\u2010like self\u2010assembled structures was found to occur above a relatively low critical aggregation concentration (CAC) by the bolaforms. The results of calorimetric measurements demonstrated thermodynamically favorable aggregation in water. Interaction studies of the cationic bolaforms with the calf thymus DNA revealed stronger binding of C\n                    <jats:sub>12<\/jats:sub>\n                    (DDUPAC)\n                    <jats:sub>2<\/jats:sub>\n                    in comparison to C\n                    <jats:sub>12<\/jats:sub>\n                    (DUAC)\n                    <jats:sub>2<\/jats:sub>\n                    , which explained higher in\u2005vitro gene transfection efficiency of C\n                    <jats:sub>12<\/jats:sub>\n                    (DDUPAC)\n                    <jats:sub>2<\/jats:sub>\n                    than C\n                    <jats:sub>12<\/jats:sub>\n                    (DUAC)\n                    <jats:sub>2<\/jats:sub>\n                    . Both bolaforms interact weakly with the bovine serum albumin protein. MTT\u2010based in\u2005vitro cytotoxicity assay was performed and both bolaforms were found to have marginal cytotoxicity. Further, both bolaforms exhibit advantageous antibacterial activity against E. coli and potent antifungal activity against Fusarium oxysporum at high dosages.\n                  <\/jats:p>","DOI":"10.1002\/asia.202401582","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T08:12:48Z","timestamp":1737015168000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Novel Cationic Bolaamphiphiles for Protein and DNA Binding, Gene Delivery, and Antimicrobial Applications"],"prefix":"10.1002","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8444-9018","authenticated-orcid":false,"given":"Pabitra","family":"Mondal","sequence":"first","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology Kharagpur  Kharagpur 721302 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6102-0924","authenticated-orcid":false,"given":"Sadhana","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Indian Institute of Technology Kharagpur  Kharagpur 721302 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6776-6478","authenticated-orcid":false,"given":"Dipanwita","family":"Patra","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Calcutta  Kolkata 700019 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5446-9582","authenticated-orcid":false,"given":"Somdeb","family":"Bose Dasgupta","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Indian Institute of Technology Kharagpur  Kharagpur 721302 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8357-5560","authenticated-orcid":false,"given":"Joykrishna","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology Kharagpur  Kharagpur 721302 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,24]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr030602b"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00408a021"},{"key":"e_1_2_8_3_1","first-page":"875","volume":"255","author":"Liu Y.","year":"2013","journal-title":"Adv. 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