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However, its clinical application has been significantly restricted due to several drawbacks, including poor water solubility, nonselective toxicity, drug resistance, and inherent instability. To address some of these challenges and to enhance the stability, solubility, and biological efficacy of CPT, in this work, we have attempted to explore nanoparticulate delivery strategies using nanostructured lipid carriers (NLCs) for the targeted delivery of CPT. We have employed L\u2010cysteine\u2010 and L\u2010cystine\u2010derived lipids, C<jats:sub>12<\/jats:sub>\u2010cys\u2010C<jats:sub>12<\/jats:sub> and [C<jats:sub>12<\/jats:sub>\u2010cys]<jats:sub>2<\/jats:sub>, along with isopropyl palmitate, as a co\u2010liquid lipid to produce surface\u2010modified NLCs. L\u2010cystine was incorporated into the lipid structure in order to exploit its redox\u2010sensitive disulfide (S\u2500S) bond for stimuli\u2010responsive delivery of CPT. Sulfur\u2010containing L\u2010cysteine and its oxidized form L\u2010cystine containing S\u2500S bond is expected to enhance anticancer drug delivery by offering improved therapeutic efficacy and biocompatibility over conventional lipids. This was confirmed by the glutathione (GSH) assay. The biocompatibility of NLC formulations was determined by in vitro MTT assay. It has been shown that compared to free CPT and cysteinelated NLC\u2010CPT, the cystinelated NLC\u2010CPT is more effective in killing both human hepatoma (HuH7) and colon carcinoma (Caco\u20102) cells.<\/jats:p>","DOI":"10.1002\/asia.202500180","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T05:04:27Z","timestamp":1749531867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Camptothecin\u2010loaded Surface\u2010modified Nanostructured Lipid Carriers Consisting of L\u2010Cysteine\u2010 and L\u2010Cystine\u2010derived Lipids: Physicochemical Characterization and In Vitro Evaluation"],"prefix":"10.1002","volume":"20","author":[{"given":"Swagata","family":"Patra","sequence":"first","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology Kharagpur  Kharagpur 721302 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipanwita","family":"Patra","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Calcutta  Kolkata 700019 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8357-5560","authenticated-orcid":false,"given":"Joykrishna","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology Kharagpur  Kharagpur 721302 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9BM00907H"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.8b00737"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5BM00002E"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9BM00407F"},{"key":"e_1_2_10_5_1","first-page":"14","author":"Xu X.","year":"2018","journal-title":"Small"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4103\/0250-474X.84590"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0987(02)00245-2"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(01)00227-9"},{"key":"e_1_2_10_9_1","volume":"1","author":"Cassano R.","year":"2019","journal-title":"Int. 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