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This study aimed to develop a durable BHV material using decellularized bovine pericardium (BP) and alternative cross\u2010linking strategies. BP was decellularized using a combination of sodium deoxycholate (SDC) and Triton X\u2010100 (TX), which removed cellular components while preserving the extracellular matrix (ECM), as confirmed by histological, DNA quantification, and biochemical analyses. The impact of glutaraldehyde, glyoxal, tannic acid, and catechin cross\u2010linking on calcification propensity and mechanical properties was evaluated. Glyoxal\u2010cross\u2010linked BP, further capped with 1%\n                    <jats:sc>l<\/jats:sc>\n                    \u2010glutamic acid, demonstrated superior resistance to calcification while maintaining mechanical properties comparable to standard glutaraldehyde treatment. These results suggest that SDC\u2010TX decellularized, glyoxal\u2010cross\u2010linked BP with\n                    <jats:sc>l<\/jats:sc>\n                    \u2010glutamic acid capping presents a promising strategy for enhancing BHV durability and biocompatibility, offering a potential alternative to conventional glutaraldehyde.\n                  <\/jats:p>","DOI":"10.1002\/asia.202500652","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T10:32:43Z","timestamp":1753093963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Combinatorial Glyoxal Cross\u2010Linking and Glutamic Acid Capping for Mitigating Calcification in Bovine Pericardium"],"prefix":"10.1002","volume":"20","author":[{"given":"Dinesh","family":"Annamalai","sequence":"first","affiliation":[{"name":"Biochemistry and Biotechnology Laboratory CSIR\u2010Central Leather Research Institute (CSIR\u2010CLRI)  Sardar Patel Road, Adyar Chennai 600020 India"},{"name":"Department of Leather Technology, Alagappa College of Technology (Housed at CSIR\u2010Central Leather Research Institute) Anna University  Chennai 600020 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiyaneshwar","family":"Ramamoorthy","sequence":"additional","affiliation":[{"name":"Biochemistry and Biotechnology Laboratory CSIR\u2010Central Leather Research Institute (CSIR\u2010CLRI)  Sardar Patel Road, Adyar Chennai 600020 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes Mary","family":"Soloman","sequence":"additional","affiliation":[{"name":"Biochemistry and Biotechnology Laboratory CSIR\u2010Central Leather Research Institute (CSIR\u2010CLRI)  Sardar Patel Road, Adyar Chennai 600020 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun","family":"Gopinath","sequence":"additional","affiliation":[{"name":"Biochemistry and Biotechnology Laboratory CSIR\u2010Central Leather Research Institute (CSIR\u2010CLRI)  Sardar Patel Road, Adyar Chennai 600020 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dineshkumar","family":"Masilamani","sequence":"additional","affiliation":[{"name":"Biochemistry and Biotechnology Laboratory CSIR\u2010Central Leather Research Institute (CSIR\u2010CLRI)  Sardar Patel Road, Adyar Chennai 600020 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarasa","family":"Bharathi","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; 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