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When combined with bioinformatics, VUVCD enables detailed determination of protein secondary\u2010structure contents, segment numbers, and sequence distributions, while coupling with linear dichroism and shear flow techniques provides insights into the orientations of secondary structures. These analytical approaches have been applied to the structural characterization of membrane\u2010bound \u03b1\u2010synuclein (\u03b1S), a protein which transforms into amyloid fibrils associated with Parkinson's disease, successfully revealing the molecular mechanism of \u03b1S fibril\u2010formation on membranes. Furthermore, time\u2010resolved measurements using microfluidic device allow direct observation of protein structural dynamics during membrane interactions. 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