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Both BCN and BCA molecules were characterized using various spectroscopic methods, and their photophysical properties were systematically examined through UV\u2013visible and both steady\u2010state and time\u2010resolved fluorescence spectroscopy. Screening of different anions demonstrated that both BCN and BCA probes exhibit selective sensitivity toward cyanide, as evidenced by distinct changes in their UV\u2013visible and fluorescence emission behaviors. Notably, upon interaction with cyanide, the original yellow\/orange coloration of the probe solution transitions to a colorless state, a change that is discernible to the naked eye. This remarkable cyanide detection mechanism is attributed to the nucleophilic addition of cyanide ions. The detection limits for cyanide sensing were determined to be 0.22\u00a0\u00d7\u00a010\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u00a0M and 0.15\u00a0\u00d7\u00a010\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u00a0M for the BCN and BCA molecular probes, respectively. This establishes a broader applicability of these probes for the detection of cyanide in real samples, such as tapioca\/cassava. Additionally, the study introduced prototype testing strips for the qualitative analysis of cyanide. The\u00a0Fluorescence microscopy imaging further indicated that the BCN dye exhibited high permeability in the Gram\u2010negative bacterium\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    ATCC 19606.\n                  <\/jats:p>","DOI":"10.1002\/asia.70324","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T10:14:56Z","timestamp":1758536096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cyanide Sensing and Bioimaging Applications of Dicyanomethylene\u20104H\u2010Benzopyran Based Electron Donor\u2010Acceptor Molecules"],"prefix":"10.1002","volume":"21","author":[{"given":"Sudeena","family":"Nadendla","sequence":"first","affiliation":[{"name":"Department of Chemistry Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjeev","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Chemistry Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suma","family":"C.S.","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amritha","family":"Ajayakumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madikonda Ashok","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margandan","family":"Bhagiyalakshmi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi Kumar","family":"Kanaparthi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Central University of Kerala  Tejaswini Hills, Periye PO Kasaragod Kerala 671 325 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"e_1_2_9_1_1","volume-title":"StatPearls","author":"Shrimanker I.","year":"2025"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V49.3.345.345"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/es300095k"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2011.06.003"},{"key":"e_1_2_9_5_1","first-page":"87","volume":"28","author":"Arroyo R. 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