{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T05:00:46Z","timestamp":1784869246688,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["aces.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Chemistry \u0097 An Asian Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Early detection of pancreatic disease requires non\u2010invasive, highly sensitive biomarker detection. Here, we report a fluorescence\u2010based method for rapid, ultrasensitive detection of urinary trypsin without sample pretreatment. A \u03c0\u2010extended pyrene fluorescent probe functionalized with four carboxylate groups was designed to facilitate selective interaction and micellar aggregation with protamine, a trypsin\u2010cleavable substrate. Protamine\u2010induced aggregation results in amplified fluorescence quenching, which is efficiently reversed by trypsin\u2010mediated proteolysis. Trypsin cleavage reduces the arginine\u2010rich positive charge density and length of protamine, disassembling the compound\n                    <jats:bold>1<\/jats:bold>\n                    \u2010protamine aggregates and recovering monomeric fluorescence, enabling highly sensitive detection. The sensing platform achieved a limit of detection (LOD) of 2.0\u00a0ng\/mL in 10% human urine, highlighting its strong analytical performance. The developed sensor exhibited excellent selectivity and enabled reliable quantification of trypsin at sub\u2010microgram levels (0.03\u20130.7\u00a0\u00b5g\/mL) in 10% diluted human urine, making it suitable for early\u2010stage pancreatic disease screening. Its clinical applicability was further validated in the presence of acute pancreatitis (AP)\u2010inducing factors, including aspirin, ibuprofen, dexibuprofen, acetaminophen, 6\u2010mercaptopurine, as well as ethanol and its metabolites (acetaldehyde and acetate). In addition, urine samples were successfully analyzed using a smartphone\u2010integrated fluorescence detection system, highlighting its practicality and clinical potential for early pancreatic disease diagnosis.\n                  <\/jats:p>","DOI":"10.1002\/asia.70557","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T17:03:16Z","timestamp":1768410196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasensitive Fluorescent Detection of Urinary Trypsin for Early Pancreatic Disease Diagnosis: Toward Smartphone\u2010Assisted Analysis"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6729-7557","authenticated-orcid":false,"given":"Minwoo","family":"Han","sequence":"first","affiliation":[{"name":"Department of Chemistry Daegu University  Gyeongsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juyeon","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Chemistry Daegu University  Gyeongsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-1993","authenticated-orcid":false,"given":"Seoung Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry Daegu University  Gyeongsan Republic of 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