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Here, we report an autistic patient with an intragenic mutation of\n                    <jats:italic>FRK<\/jats:italic>\n                    and two other autism\u2010related genes. Circumventing the barrier of murine model studies, we generated the CRISPR\/Cas9\u2010engineered\n                    <jats:italic>frk<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    zebrafish. We found that\n                    <jats:italic>frk<\/jats:italic>\n                    \u2010knockout led to early developmental abnormalities.\n                    <jats:italic>Frk<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    fish exhibited autism\u2010like behavior, including impaired social communication, repetitive stereotypic behavior, altered anxiety level, and cognitive dysfunction in both larvae and adults, which could be rescued by the transgenic neuron\u2010specific re\u2010expression of\n                    <jats:italic>frk<\/jats:italic>\n                    . GO and KEGG analysis of RNA\u2010sequencing data found that the\n                    <jats:italic>frk<\/jats:italic>\n                    \u2010knockout\u2010induced DEGs were mainly concentrated in processes and functions related to cell metabolism. PPI network analysis of the detected DEGs suggested that the\n                    <jats:italic>cyp24a1\/tp53<\/jats:italic>\n                    pathway may play a key role in\n                    <jats:italic>frk<\/jats:italic>\n                    \u2010knockout\u2010induced autism. Furthermore, we found that the numbers of BrdU\n                    <jats:sup>+<\/jats:sup>\n                    cells were significantly increased in the\n                    <jats:italic>frk<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    larval brains. Inhibition of Cyp24a1 or activation of tp53 reduced cell proliferation and partially ameliorated social impairments in\n                    <jats:italic>frk<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    zebrafish. Overall, our work established an autism model of\n                    <jats:italic>frk<\/jats:italic>\n                    \u2010knockout with an assessable behavior phenotype in zebrafish and provided key insights into cell proliferation and the influence of the\n                    <jats:italic>cyp24a1\/tp53<\/jats:italic>\n                    pathway\u2010regulated cell proliferation on\n                    <jats:italic>frk<\/jats:italic>\n                    \u2010knockout\u2010induced autism\u2010like behaviors.\n                  <\/jats:p>","DOI":"10.1002\/aur.70310","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T03:01:18Z","timestamp":1783566078000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zebrafish\n                    <i>frk<\/i>\n                    Gene Knockout Shows Social Impairments Relevant to Autism and Delays Brain Development by Increasing Cell Proliferation"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6559-8128","authenticated-orcid":false,"given":"Xueting","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Psychology, Zhejiang University School of Medicine Children's Hospital National Clinical Research Center for Child Health  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Psychology, Zhejiang University School of Medicine Children's Hospital National Clinical Research Center for Child Health  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingchao","family":"Ying","sequence":"additional","affiliation":[{"name":"Department of Psychology, Zhejiang University School of Medicine Children's Hospital National Clinical Research Center for Child Health  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Binhong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Biobank Center, The Children's Hospital Zhejiang University School of Medicine National Clinical Research Center for Child Health  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Psychology, Zhejiang University School of Medicine Children's Hospital National Clinical Research Center for Child Health  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zou","sequence":"additional","affiliation":[{"name":"Eye Center of the Second Affiliated Hospital, School of Medicine Zhejiang University  Hangzhou China"},{"name":"The Institute of Translational Medicine Zhejiang University  Hangzhou China"},{"name":"Zhejiang Provincial Key Lab of Ophthalmology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3147-6514","authenticated-orcid":false,"given":"Kewen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Psychology, Zhejiang University School of Medicine Children's Hospital National Clinical Research Center for Child Health  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2013-101951"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2007.02.009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21974"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2009.07.009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro-2014-0056"},{"key":"e_1_2_10_7_1","first-page":"1347","article-title":"Rak, a Novel Nuclear Tyrosine Kinase Expressed in Epithelial Cells","volume":"5","author":"Cance W. 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