{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:14:02Z","timestamp":1791868442987,"version":"4.3.4"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,3,29]],"date-time":"2011-03-29T00:00:00Z","timestamp":1301356800000},"content-version":"vor","delay-in-days":87,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antibodies produced in animals vaccinated using live attenuated vaccines against <jats:italic>Brucella<\/jats:italic> spp. are indistinguishable using current conventional serological tests from those produced in infected animals. One potential approach is to develop marker vaccines in which specific genes have been deleted from parental vaccine strains that show good immunogenicity and vaccine efficacy. Corresponding methods of detection for antibodies raised by the marker vaccine should also be developed. A specific fragment of the <jats:italic>bp26<\/jats:italic> gene of <jats:italic>Brucella melitensis<\/jats:italic> M5\u201390 was cloned into vector pQE32 to construct the recombinant plasmid (pQE32\u2010r\u0394bp26). It was used to transform <jats:italic>Escherichia coli<\/jats:italic> M15 (pREP4) host cells, which expressed the r\u0394bp26 protein. Subsequently, the recombinant protein was purified by immobilized metal affinity chromatography and size\u2010exclusion chromatography. The results of sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis showed that the purified r\u0394bp26 protein was represented by only one band, with a molecular weight of 14 kDa, and it showed good antigenic specificity on western blot and enzyme\u2010linked immunosorbent assay (ELISA). The purified r\u0394bp26 protein was intended to be used as an antigen to develop a novel ELISA to differentiate animals vaccinated with bp26 mutants of <jats:italic>Brucella<\/jats:italic> spp. from those infected naturally and those vaccinated with the parental vaccine strains.<\/jats:p>","DOI":"10.1002\/bab.11","type":"journal-article","created":{"date-parts":[[2011,4,4]],"date-time":"2011-04-04T08:35:00Z","timestamp":1301906100000},"page":"32-38","source":"Crossref","is-referenced-by-count":13,"title":["Expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of <i>Brucella melitensis<\/i> M5\u201090: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats"],"prefix":"10.1002","volume":"58","author":[{"given":"Liu","family":"Wen\u2010xing","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Sen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Zu\u2010jian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Wei\u2010ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Lin\u2010tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Fang\u2010kun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Rong\u2010hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bu","family":"ZHi\u2010gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiang\u2010rui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,29]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02622.x"},{"key":"e_1_2_8_3_2","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.smallrumres.2006.01.012","volume":"70","author":"Gupta V. 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