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But because of its poor solubilization in water and the emergence of resistant strains against this antimycotic drug, we aimed at devising a unique microemulsion drug delivery system for fluconazole against candidiasis. A clear oil\u2010in\u2010water microemulsion system, consisting of clove oil as oil phase, Tween 20 as surfactant, and water as aqueous phase was developed using a ternary phase diagram. Physicochemical characterization was done to understand the internal physicochemical state. The bulk drug, fluconazole, that measured several microns in length was reduced to a 10\u201365 nm range with no means of high\u2010energy methods as confirmed by transmission electron microscopy. The very small and uniform spherical structure of the drug\u2010loaded microemulsion system could be of high impact to the biological system as the efficacy of fluconazole is greatly improved when compared with its conventional bulk form. The optimized microemulsion exhibited significantly higher antifungal activity at a minimum concentration (8\u00a0\u00b5g\/ml) of fluconazole as examined by fluorescence and scanning electron microscopy. Thus, our report discloses an excellent oral drug delivery system.<\/jats:p>","DOI":"10.1002\/bab.1116","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T19:20:38Z","timestamp":1365103238000},"page":"417-429","source":"Crossref","is-referenced-by-count":17,"title":["Improved efficacy of fluconazole against candidiasis using bio\u2010based microemulsion technique"],"prefix":"10.1002","volume":"60","author":[{"given":"M. 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