{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:01:21Z","timestamp":1773885681125,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human <jats:italic><jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1<\/jats:italic> (placenta\u2010specific 1) is a new member of cancer\u2013testis antigens with 212 amino acids, and its expression is restricted to placenta and at much lower levels to testis. Recently, ectopic expression of the <jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1 transcript has been demonstrated in a wide range of human tumors and cancer cell lines with a proposed function in tumor cell growth. No monoclonal anti\u2010<jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1 antibody applicable to immunohis\u2010tochemical staining is available so far. To better understand the <jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1 expression and localization, we aimed to produce monoclonal antibodies (m<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>bs) against the extracellular region of <jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1. Mice were immunized with a synthetic peptide corresponding to the <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\u2010terminal 11 amino acids of <jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1 conjugated with a carrier protein. Hybridomas were produced by standard protocol and screened for positive reactivity by enzyme\u2010linked immunosorbent assay. Reactivity of final two clones was then assessed by <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blotting (WB), immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>), and immunocytochemistry (<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>). Both clones showed a specific immunostaining pattern in human term placenta as the positive control. Reactivity was mostly localized to the cytoplasm of syncytiotrophoblasts. One of the clones showed an excellent staining signal in breast, ovary, and prostate cancer cell lines. Importantly, no reactivity was observed with human lymph node cells or prostate. None of the m<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>bs were able to detect <jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1 in <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blot. Based on the present results, these m<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>bs can be used for detection of <jats:styled-content style=\"fixed-case\">PLAC<\/jats:styled-content>1 in <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> techniques.<\/jats:p>","DOI":"10.1002\/bab.1177","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T00:53:14Z","timestamp":1384822394000},"page":"363-369","source":"Crossref","is-referenced-by-count":23,"title":["Immunohistochemical characterization of novel murine monoclonal antibodies against human placenta\u2010specific 1"],"prefix":"10.1002","volume":"61","author":[{"given":"Roya","family":"Ghods","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine School of Advanced Medical Technologies Tehran University of Medical Sciences  Tehran Iran"},{"name":"Monoclonal Antibody Research Center Avicenna Research Institute ACECR  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Hossein","family":"Ghahremani","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine School of Advanced Medical Technologies Tehran University of Medical Sciences  Tehran Iran"},{"name":"Department of Pharmacology\u2010Toxicology Faculty of Medicine Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Darzi","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Research Center Avicenna Research Institute ACECR  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad\u2010Reza","family":"Mahmoudi","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Research Center Avicenna Research Institute ACECR  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omid","family":"Yeganeh","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Research Center Avicenna Research Institute ACECR  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali Ahmad","family":"Bayat","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Research Center Avicenna Research Institute ACECR  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parvin","family":"Pasalar","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine School of Advanced Medical Technologies Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmood","family":"Jeddi\u2010Tehrani","sequence":"additional","affiliation":[{"name":"Monoclonal Antibody Research Center Avicenna Research Institute ACECR  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir\u2010Hassan","family":"Zarnani","sequence":"additional","affiliation":[{"name":"Nanobiotechnology Research Center Avicenna Research Institute ACECR  Tehran Iran"},{"name":"Immunology Research Center Iran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6302"},{"key":"e_1_2_8_3_1","first-page":"18","volume":"7","author":"Silva W. 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