{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T05:40:36Z","timestamp":1696570836333},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,4,21]],"date-time":"2014-04-21T00:00:00Z","timestamp":1398038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific Research Program of Shanghai Research Institute of Chemical Industry","award":["1806A-1"],"award-info":[{"award-number":["1806A-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Rapid and efficient extraction of bioactive compounds from biomass for sample analysis presents one of the major challenges in high\u2010productive strain screening and bioprocess development. In this work, a high\u2010throughput method based on a new setup was developed for the extraction of <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/bab1178-math-0004.png\" xlink:title=\"urn:x-wiley:08854513:bab1178:equation:bab1178-math-0004\" \/>\u2010carotene from <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>lakeslea trispora<\/jats:italic>. After the cell was completely disrupted by high\u2010throughput machinery grinding, <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/bab1178-math-0005.png\" xlink:title=\"urn:x-wiley:08854513:bab1178:equation:bab1178-math-0005\" \/>\u2010carotene was completely extracted by chloroform and dimethylsulfoxide (1:1, v\/v). According to the experimental results, the newly developed high\u2010throughput extraction (<jats:styled-content style=\"fixed-case\">HTPE<\/jats:styled-content>) method could simultaneously process 96 samples within 30\u00a0Min, and only 5\u2010mL solvent was used for each sample. The sufficient extraction of <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/bab1178-math-0006.png\" xlink:title=\"urn:x-wiley:08854513:bab1178:equation:bab1178-math-0006\" \/>\u2010carotene by the <jats:styled-content style=\"fixed-case\">HTPE<\/jats:styled-content> method, which was confirmed by the conventional reference extraction method, further demonstrated that the newly developed <jats:styled-content style=\"fixed-case\">HTPE<\/jats:styled-content> method was more efficient and economical than the methods developed previously for the extraction of <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/bab1178-math-0007.png\" xlink:title=\"urn:x-wiley:08854513:bab1178:equation:bab1178-math-0007\" \/>\u2010carotene.<\/jats:p>","DOI":"10.1002\/bab.1178","type":"journal-article","created":{"date-parts":[[2013,11,19]],"date-time":"2013-11-19T00:53:16Z","timestamp":1384822396000},"page":"446-452","source":"Crossref","is-referenced-by-count":1,"title":["High\u2010throughput extraction of \u2010carotene from <i><scp>B<\/scp>lakeslea trispora<\/i> based on a newly developed setup"],"prefix":"10.1002","volume":"61","author":[{"given":"Hong\u2010Wei","family":"Dong","sequence":"first","affiliation":[{"name":"Shanghai Research Institute of Chemical Industry  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Quan","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Research Institute of Chemical Industry  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Gang","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Research Institute of Chemical Industry  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Yang","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Research Institute of Chemical Industry  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Sen","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Research Institute of Chemical Industry  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Jiang","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Metabolism School of Life Sciences and Biotechnology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398209527357"},{"key":"e_1_2_7_3_1","first-page":"183","volume":"47","author":"Isler O.","year":"1956","journal-title":"Mitt. 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