{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T10:40:03Z","timestamp":1696588803525},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T00:00:00Z","timestamp":1395705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heterologous expression is an efficient alternative to conventional extraction to produce a specific <jats:italic>Buthus martensii<\/jats:italic> <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>arsch (<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>) peptide. In this work, <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 was successfully expressed in <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>scherichia coli<\/jats:italic> after genetic codon optimization, but <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 content was &lt;6% of total cellular protein. To improve <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 expression, a trc promoter library with a wide relative strength was constructed, and three promoters, <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><jats:sub>pJF136<\/jats:sub> (0.55), <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><jats:sub>pJF325<\/jats:sub> (1.29), and <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><jats:sub>pJF288<\/jats:sub> (2.31), were selected to control <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 expression. A higher <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 expression (&gt;13.9% of total protein) was obtained using a stronger promoter, <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><jats:sub>pJF325<\/jats:sub>. Furthermore, a maximum <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 content (&gt;21.7% of total protein) was obtained by combining promoter <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><jats:sub>pJF325<\/jats:sub> and three copies of the <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1<\/jats:italic> gene. The yield of the purified <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>m<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>1 achieved 196.74\u00a0mg L<jats:sup>\u22121<\/jats:sup> in <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>. coli<\/jats:italic> <jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>21(<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>3) p<jats:styled-content style=\"fixed-case\">JF<\/jats:styled-content>431, which was improved 2.09\u2010fold compared with the control. This was the highest reported production of scorpion peptides in <jats:italic><jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>. coli<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/bab.1194","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T11:31:12Z","timestamp":1387884672000},"page":"466-473","source":"Crossref","is-referenced-by-count":1,"title":["Significant expression of a <scp>C<\/scp>hinese scorpion peptide, <scp>B<\/scp>m<scp>K<\/scp>1, in <i><scp>E<\/scp>scherichia coli<\/i> through promoter engineering and gene dosage strategy"],"prefix":"10.1002","volume":"61","author":[{"given":"Jianfeng","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Synthetic Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences  Shanghai People's Republic of China"},{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiqiang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingying","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences  Shanghai People's Republic of China"},{"name":"State Key Laboratory of Bioreactor Engineering East China University of Science and Technology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences  Shanghai People's Republic of 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