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For formulating <jats:italic>Bacillus fastidious<\/jats:italic> uricase, succinimidyl carbonate of m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\u20105000 (<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>5k) and succinimidyl succinate of m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>\u20105000 (<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>5k) were compared. <jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>5k possessed higher purity, comparable reaction rate constant with glycine but lower hydrolysis rate, and stronger effectiveness to modify amino groups. The uricase possessed two types of amino groups bearing a 25\u2010fold difference in reactivity with <jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>5k or <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>5k at pH 9.2. Oxonate and xanthine concentration\u2010dependently protected the bacterial uricase from inactivation during <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>ylation. With <jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>5k at a molar ratio of 200 to uricase subunits and oxonate of 50\u00a0\u00b5M, the <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>ylated uricase (1) retained about 73% of the original activity, (2) displayed about 10% reactivity to rabbit anti\u2010sera recognizing the native uricase, (3) elicited IgG in rats accounting for about 5% of that by the native uricase, (4) exhibited circulation half\u2010life time of about 25\u00a0H in cock plasma <jats:italic>in vivo<\/jats:italic>, and (5) concurrently maintained uric acid at lowered levels for over 20\u00a0H. Hence, <jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>ylation with <jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>\u2010m<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content> under the protection of a competitive inhibitor was a practical approach to formulation of the bacterial uricase; protection of enzymes by competitive inhibitors during PEGylation may have universal significance.<\/jats:p>","DOI":"10.1002\/bab.1215","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T09:23:37Z","timestamp":1392197017000},"page":"683-690","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of modification of a bacterial uricase with <i><scp>N<\/scp><\/i>\u2010hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)"],"prefix":"10.1002","volume":"61","author":[{"given":"Chun","family":"Zhang","sequence":"first","affiliation":[{"name":"Unit for Analytical Probes 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