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The variable domain of heavy\u2010chain antibodies (VHH) has unique features, such as the ability to identify and bind specifically to target epitopes and ease of production in bacteria and yeast. The <jats:italic>Pichia pastoris<\/jats:italic> is suitable for expression of recombinant antibody fragments. Disulfide bond formation and correct folds of protein with a high yield are some of the advantages of this eukaryotic host. In this study, we have expressed and purified the camelid VHH against BoNT\/E in <jats:italic>P. pastoris<\/jats:italic>. The final yield of <jats:italic>P. pastoris<\/jats:italic>\u2010expressed antibody was estimated to be 16 mg\/l, which is higher than that expressed by <jats:italic>Escherichia coli<\/jats:italic>. The nanobody expressed in <jats:italic>P. pastoris<\/jats:italic> neutralized 4LD<jats:sub>50<\/jats:sub> of the BoNT\/E upon i.p. injection in 25% of mice. The nanobody expressed in <jats:italic>E. coli<\/jats:italic> extended the mice's survival to 1.5\u2010fold compared to the control. This experiment indicated that the quality of expressed protein in the yeast is superior to that of the bacterial expression. Favorable protein folding by <jats:italic>P<\/jats:italic>. <jats:italic>pastoris<\/jats:italic> seems to play a role in its better toxin\u2010binding property.<\/jats:p>","DOI":"10.1002\/bab.1226","type":"journal-article","created":{"date-parts":[[2014,3,26]],"date-time":"2014-03-26T19:06:59Z","timestamp":1395860819000},"page":"200-205","source":"Crossref","is-referenced-by-count":33,"title":["Camelid\u2010derived heavy\u2010chain nanobody against <i>Clostridium botulinum<\/i> neurotoxin E in <i>Pichia pastoris<\/i>"],"prefix":"10.1002","volume":"63","author":[{"given":"Roghayyeh","family":"Baghban","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Basic Sciences Shahed University  Tehran\u2010Qom Express Way Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Latif Mousavi","family":"Gargari","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Basic Sciences Shahed University  Tehran\u2010Qom Express Way Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoumeh","family":"Rajabibazl","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Faculty of Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahram","family":"Nazarian","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Faculty of Sciences Imam Hosein University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Bakherad","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Basic Sciences Shahed University  Tehran\u2010Qom Express Way Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2009.09.008"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.07.002"},{"key":"e_1_2_6_4_1","first-page":"181","volume":"9","author":"Mousavi Gargari S. 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