{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T07:12:36Z","timestamp":1696662756119},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,7,14]],"date-time":"2014-07-14T00:00:00Z","timestamp":1405296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adenosine deaminase (<jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content>) deficiency, where a deleterious mutation in the <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> gene of patients results in a dysfunctional immune system, is ultimately caused by an absence of <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content>. Over the last 25 years the disease has been treated with <jats:styled-content style=\"fixed-case\">PEG\u2010ADA<\/jats:styled-content>, made from purified bovine <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> coupled with polyethylene glycol (<jats:styled-content style=\"fixed-case\">PEG<\/jats:styled-content>). However, it is thought that an enzyme replacement therapy protocol based on recombinant human <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> would probably be a more effective treatment. With this end in mind, a human <jats:styled-content style=\"fixed-case\">ADA cDNA<\/jats:styled-content> was inserted into plant expression vectors used to transform tobacco plant cell suspensions. Transgenic calli expressing constructs containing apoplast\u2010directing signals showed significantly higher levels of recombinant <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> expression than calli transformed with cytosolic constructs. The most significant <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> activities, however, were measured in the media of transgenic cell suspensions prepared from high expressing transformed calli: where incorporation of a signal for arabinogalactan addition to <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> led to a recombinant protein yield of approximately 16\u00a0mg\u00a0L<jats:sup>\u22121<\/jats:sup>, a 336\u2010fold increase over <jats:styled-content style=\"fixed-case\">ADA<\/jats:styled-content> produced by cell suspensions transformed with a cytosolic construct.<\/jats:p>","DOI":"10.1002\/bab.1240","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T17:58:27Z","timestamp":1405447107000},"page":"87-93","source":"Crossref","is-referenced-by-count":5,"title":["High\u2010yield production of apoplast\u2010directed human adenosine deaminase in transgenic tobacco <scp>BY<\/scp>\u20102 cell suspensions"],"prefix":"10.1002","volume":"62","author":[{"given":"Sanjeewa","family":"Singhabahu","sequence":"first","affiliation":[{"name":"Human Genetics Unit Faculty of Medicine University of Colombo  Kynsey Road Colombo 8 Sri Lanka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"George","sequence":"additional","affiliation":[{"name":"School of Health Sport and Bioscience University of East London  Water Lane London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bringloe","sequence":"additional","affiliation":[{"name":"School of Health Sport and Bioscience University of East London  Water Lane London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2006.12.009"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.07.006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-06-189209"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-012-9676-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1027"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0926-6690(92)90025-Q"},{"key":"e_1_2_6_8_1","first-page":"9","volume":"5","author":"Showalter A.M.","year":"1993","journal-title":"Plant Cell"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc970107k"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21407"},{"key":"e_1_2_6_11_1","unstructured":"Pfizer Press Releases. 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